THE INFLUENCE OF THE KLEBSIELLA-PNEUMONIAE REGULATORY GENE NIFL UPON THE TRANSCRIPTIONAL ACTIVATOR PROTEIN NIFA

被引：22
作者：
MORETT, E [1 ]
KREUTZER, R [1 ]
CANNON, W [1 ]
BUCK, M [1 ]
机构：
[1] UNIV SUSSEX,AFRC,IPSR,BRIGHTON BN1 9RQ,E SUSSEX,ENGLAND
来源：
MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 08期
关键词：
D O I：
10.1111/j.1365-2958.1990.tb00704.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The influence of the Klebsiella pneumoniae nifL gene product upon the interaction of the transcriptional activator protein NifA with the nifH promoter has been examined using in vivo dimethylsulphate ‘footprinting’. Binding of NifA to the upstream activator sequence (UAS) of the nifH promoter in the presence of the NifL protein was observed under nitrogen‐limiting growth conditions. Growth in the presence of NH+4 or addition of NH+4 to nitrogen‐limited cells diminished the interaction of NifA with the UAS when NifL was present. Repression of nif transcription by NifL may therefore involve an interaction between NifL and NifA which reduces the affinity of NifA for the UAS. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：1253 / 1258
页数：6
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