REGIONAL LOCALIZATION OF THE HUMAN-G PROTEIN-ALPHA-I2 (GNAI2) GENE - ASSIGNMENT TO 3P21 AND A RELATED SEQUENCE (GNAI2L) TO 12P12-P13

G1α proteins, members of the G protein signal transduction family, include a small number of polypeptides: Giα1 (GNAI1), Giα2 (GNAI2), and Giα3 (GNAI3). A cDNA for the human GNAI2 gene has been isolated from a human T-cell library and is mapped by chromosomal in situ hybridization to the short arm of chromosome 3 at 3p21. A related sequence, GNAI2L, is mapped by in situ hybridization to the short arm of chromosome 12 at p12-p13. These mapping results are further supported by amplification of GNAI2-specific sequences in a monochromosomal human/rodent somatic cell hybrid containing only human chromosome 3. Of note, these assignments are to chromosome regions in which other G proteins reside. Localization of GNAI2 to 3p21 is of great interest as this region of the short arm of chromosome 3 is frequently involved in rearrangements in various human tumors. © 1992.