NADPH-CYTOCHROME-P-450 OXIDOREDUCTASE GENE ORGANIZATION CORRELATES WITH STRUCTURAL DOMAINS OF THE PROTEIN

被引：74

作者：

PORTER, TD ^{[1
]}

BECK, TW ^{[1
]}

KASPER, CB ^{[1
]}

机构：

[1] UNIV WISCONSIN,MCARDLE LAB CANC RES,MADISON,WI 53706

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 42期

关键词：

D O I：

10.1021/bi00494a009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones to rat liver NADPH-cytochrome P-450 oxidoreductase were used to isolate genomic clones from a Wistar-Furth inbred rat genomic DNA library. Fifteen exons containing the coding region and 3′-nontranslated segment of the P-450 reductase gene were identified, spanning 20 kilobases of DNA contained in 3 λ-Charon 35 clones. The organization of this single copy gene reveals a general correspondence between exons and structural domains of the protein, with the segment responsible for anchoring the reductase to the microsomal membrane and several segments involved in FMN, FAD, and NADPH binding encoded by discrete exons. © 1990, American Chemical Society. All rights reserved.

引用

页码：9814 / 9818

页数：5

共 41 条

[1] ALCOHOL-DEHYDROGENASE GENE OF DROSOPHILA-MELANOGASTER - RELATIONSHIP OF INTERVENING SEQUENCES TO FUNCTIONAL DOMAINS IN THE PROTEIN [J].

BENYAJATI, C ;

PLACE, AR ;

POWERS, DA ;

SOFER, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (05) :2717-2721

[2] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION [J].

BIGGIN, MD ;

GIBSON, TJ ;

HONG, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3963-3965

[3] GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES [J].

BLIN, N ;

STAFFORD, DW .

NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2303-2308

[4]

BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025

[5] CLONING AND STRUCTURE OF THE HUMAN ADRENODOXIN GENE [J].

CHANG, CY ;

WU, DA ;

LAI, CC ;

MILLER, WL ;

CHUNG, BC .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1988, 7 (09) :609-615

[6] EVOLUTION AND STRUCTURE OF THE FIBRINOGEN GENES - RANDOM INSERTION OF INTRONS OR SELECTIVE LOSS [J].

CRABTREE, GR ;

COMEAU, CM ;

FOWLKES, DM ;

FORNACE, AJ ;

MALLEY, JD ;

KANT, JA .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 185 (01) :1-19

[7] CHARACTERIZATION OF GLOBIN DOMAINS - HEME BINDING TO THE CENTRAL EXON PRODUCT [J].

CRAIK, CS ;

BUCHMAN, SR ;

BEYCHOK, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1384-1388

[8] GENES-IN-PIECES REVISITED [J].

GILBERT, W .

SCIENCE, 1985, 228 (4701) :823-824

[9] WHY GENES IN PIECES [J].

GILBERT, W .

NATURE, 1978, 271 (5645) :501-501

[10]

GONZALEZ FJ, 1983, J BIOL CHEM, V258, P1363

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