3-DIMENSIONAL CRYSTALS OF CYTOCHROME-C-OXIDASE FROM THERMUS-THERMOPHILUS DIFFRACTING TO 3.8 ANGSTROM RESOLUTION

被引：18
作者：
SOULIMANE, T
GOHLKE, U
HUBER, R
BUSE, G
机构：
[1] RHEIN WESTFAL TH AACHEN KLINIKUM, INST BIOCHEM, D-52057 AACHEN, GERMANY
[2] MAX PLANCK INST BIOCHEM, D-82152 MARTINSRIED, GERMANY
来源：
FEBS LETTERS | 1995年 / 368卷 / 01期
关键词：
MEMBRANE PROTEIN CRYSTALLIZATION; X-RAY DIFFRACTION; CYTOCHROME-C OXIDASE; BA(3)-OXIDASE; THERMUS THERMOPHILUS;
D O I：
10.1016/0014-5793(95)00623-H
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ba(3)-type cytochrome-c oxidase from Thermus thermophilus has been crystallized in its native form. Crystallization was achieved by the batch and the vapour diffusion sitting drop methods using polyethylene glycol monomethyl ether 2000 as precipitating agent in the presence of octyl-beta-D-thioglucoside as detergent. The crystals diffract to 3.8 Angstrom, belong to the space group P2 or P2(1) and have unit cell. dimensions of a = 80.7 Angstrom; b = 116.0 Angstrom; c = 156.9 Angstrom and beta = 104.4 degrees, The asymmetric unit contains true ba(3)-type oxidase molecules.
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页码：132 / 134
页数：3
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