FORENSIC APPLICATION OF A RAPID AND QUANTITATIVE DNA SEX TEST BY AMPLIFICATION OF THE X-Y HOMOLOGOUS GENE AMELOGENIN

被引:169
作者
MANNUCCI, A
SULLIVAN, KM
IVANOV, PL
GILL, P
机构
[1] CENT RES & SUPPORT ESTAB, FORENS SCI SERV, READING RG7 4PN, BERKS, ENGLAND
[2] UNIV GENOA, IST MED LEGALE, I-16132 GENOA, ITALY
[3] RUSSIAN ACAD SCI, VA ENGELHARDT MOLEC BIOL INST, MOSCOW 117984, RUSSIA
关键词
SEX TEST; AMELOGENIN; PCR; HLA-DQA1;
D O I
10.1007/BF01371335
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
Gender identification of forensic samples was determined by amplifying a segment of the X-Y homologous gene amelogenin. Using a single pair of primers spanning part of the first intron, 106 bp and 112 bp PCR products were generated from the X and Y homologues respectively, which were then resolved by agarose gel electrophoresis. This test enabled as little as 20 pg of DNA from severely degraded bones to be amplified and typed in a single tube reaction. Furthermore, using dye-labelled primers, it was possible to quantitate, by automated fluorescence detection, the relative yields of X and Y-specific PCR products generated from mixtures of male and female DNA. The versatility of this sex test was further demonstrated by co-amplifying with the HLA-DQA1 Amplitype kit in a combined gender/identity DNA test.
引用
收藏
页码:190 / 193
页数:4
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