SPECTROFLUORIMETRIC DETERMINATION OF TOLTERODINE TARTARATE IN PURE FORM AND PHARMACEUTICAL PREPARATION

被引：11

作者：

Nassar, Mohammed W. ^{[1
]}

Attia, Khalid A. ^{[1
]}

Abou-Seada, Hamed M. ^{[1
]}

Allam, Ahmed El-Olemy ^{[1
]}

机构：

[1] Al Azhar Univ, Fac Pharm, Dept Pharmaceut Analyt Chem, Cairo, Egypt

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH | 2013年 / 4卷 / 10期

关键词：

Tolterodine; Spectrofluorimetric; pure form; Pharmaceutical preparation;

D O I：

10.13040/IJPSR.0975-8232.4(10).3845-49

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A Simple, rapid, sensitive, accurate and precise spectrofluorimetric method was developed for the determination of tolterodine tartrate (TOL) in bulk powder and in pharmaceutical preparation. This method is based on measuring the native fluorescence of TOL in methanol at 313 nm after excitation at 285 nm using 1 ml of phosphate buffer pH 6. All variables that affect fluorescence intensity such as diluting solvents, pH, buffer and buffer volume were studied and optimized. The fluorescence-concentration plot was rectilinear over the range of 0.5-8 mu g/ ml with a lower detection limit (LOD) of 0.090 mu g/ ml and lower quantification limit (LOQ) of 0.273 mu g/ ml. The method was validated and successfully applied to the determination of TOL tablets with an average percent recovery +/- RSD% of 100.57 +/- 0.76. The obtained results were statistically compared with those of the reported method by applying ttest and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision.

引用

页码：3845 / 3849

页数：5