BIOCONVERSION OF VANILLIN INTO VANILLIC ACID BY PSEUDOMONAS-FLUORESCENS STRAIN BTP9 - REACTOR DESIGN AND PARAMETERS OPTIMIZATION

被引：7

作者：

BARE, G

DELAUNOIS, V

RIKIR, R

THONART, P

机构：

[1] Centre Wallon de Biologie Industrielle, Université de Liège, Liège, 4000, F.S.A. Gx, Sart-Tilman

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 45-6卷

关键词：

VANILLIN; VANILLIC ACID; PSEUDOMONAS FLUORESCENS; IMMOBILIZED CELL REACTOR; 2-PHASE REACTOR;

D O I：

10.1007/BF02941833

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudomonas fluorescens strain BTP9 is used as biocatalyst to produce vanillic acid from vanillin. Several two-phase reactors were investigated and compared to the corresponding one-phase systems in order to optimize this bioconversion. A water-dodecanol system was set up. High cell density and entrapment of the cells in alginate beads are two characteristics of this reactor. With this kind of reactor, vanillic acid productivity was increased (3.4 g/L/d) compared to the one-phase reactor, with a conversion rate near 80%. Vanillic acid yield and productivity strongly depended on pH, stirring rate, cell immobilization, and substrate concentration.

引用

页码：599 / 610

页数：12

共 2 条

[1] BIOCONVERSION OF VANILLIN INTO VANILLIC ACID BY PSEUDOMONAS-FLUORESCENS STRAIN BTP9 - CELL REACTORS AND MUTANTS STUDY
BARE, G
GERARD, J
JACQUES, P
DELAUNOIS, V
THONART, P
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1992, 34-5 : 499 - 510
[2] Bioconversion of ferulic acid into vanillic acid by means of a vanillate-negative mutant of Pseudomonas fluorescens strain BF13
Civolani, C
Barghini, P
Roncetti, AR
Ruzzi, M
Schiesser, A
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (06) : 2311 - 2317

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