MICROCARRIER CULTURE OF FISH CELLS AND VIRUSES IN CELL-CULTURE BIOREACTOR

被引:4
|
作者
CHEN, ZH [1 ]
CHEN, YL [1 ]
SHI, Y [1 ]
YIE, XP [1 ]
YANG, GZ [1 ]
机构
[1] FRESHWATER FISHERIES INST ZHEJIANG PROVINCE,HUZHOU 311001,PEOPLES R CHINA
关键词
FISH CELL; HEMORRHAGE VIRUS; MICROCARRIER; CELL CULTURE;
D O I
10.1139/m92-037
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This article deals with the culture of grass carp (Ctenopharyngodon idellus) lip and embryo cells on Cytodex 3 and GT-2 microcarriers in a 1.5-L cell culture bioreactor to propagate grass carp hemorrhage virus. The cells and viruses were successfully cultivated at 26-degrees-C, pH 7.0, and dissolved oxygen 40% of air saturation. The cell density achieved was as high as 7.4 x 10(6) cells/mL, and the virus titre reached 6.75 log LD50/0.5 mL from an initial 3.00 log LD50/0.5 mL. The results present broad prospects for fish virus vaccine production.
引用
收藏
页码:222 / 225
页数:4
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