MICROCARRIER CULTURE OF FISH CELLS AND VIRUSES IN CELL-CULTURE BIOREACTOR

被引：4

作者：

CHEN, ZH ^{[1
]}

CHEN, YL ^{[1
]}

SHI, Y ^{[1
]}

YIE, XP ^{[1
]}

YANG, GZ ^{[1
]}

机构：

[1] FRESHWATER FISHERIES INST ZHEJIANG PROVINCE,HUZHOU 311001,PEOPLES R CHINA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1992年 / 38卷 / 03期

关键词：

FISH CELL; HEMORRHAGE VIRUS; MICROCARRIER; CELL CULTURE;

D O I：

10.1139/m92-037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article deals with the culture of grass carp (Ctenopharyngodon idellus) lip and embryo cells on Cytodex 3 and GT-2 microcarriers in a 1.5-L cell culture bioreactor to propagate grass carp hemorrhage virus. The cells and viruses were successfully cultivated at 26-degrees-C, pH 7.0, and dissolved oxygen 40% of air saturation. The cell density achieved was as high as 7.4 x 10(6) cells/mL, and the virus titre reached 6.75 log LD50/0.5 mL from an initial 3.00 log LD50/0.5 mL. The results present broad prospects for fish virus vaccine production.

引用

页码：222 / 225

页数：4

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