FLUORESCENCE PROPERTIES OF NEUROSPORA TYROSINASE

被引：18

作者：

BELTRAMINI, M ^{[1
]}

LERCH, K ^{[1
]}

机构：

[1] UNIV ZURICH, INST BIOCHEM, CH-8028 ZURICH, SWITZERLAND

来源：

BIOCHEMICAL JOURNAL | 1982年 / 205卷 / 01期

关键词：

D O I：

10.1042/bj2050173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Some structural properties of Neurospora tyrosinase were studied by fluorescence spectroscopy. The emission spectra observed for oxy-, deoxy-, met- and apo-tyrosinase and the Co2+-substituted form are indicative of a protein containing buried tryptophan residues. By using acrylamide and iodide, part of the emission is quenched, indicating heterogeneity in the tryptophan environment. Upon binding of Cu2+ or Co2+ to apo-tyrosinase, a marked decrease of the tryptophan quantum yield is observed. A further decrease in emission intensity results from the binding of molecular O2 to the deoxy form. The fluorescent probe 8-anilinonaphthalene-1-sulfonate binds to tyrosinase only when the metal ions are removed. Reconstitution of apo-tyrosinase with Cu2+ completely displaces the probe, suggesting that 8-anilinonaphthalene-1-sulfonate binds to apo-tyrosinase at the active site. The fluorescence properties of Neurospora tyrosinase are compared with those of hemocyanin.

引用

页码：173 / 180

页数：8

共 46 条

[1] EFFECTS THAT ENVIRONMENT EXERTS ON SPECTROSCOPIC PROPERTIES OF CERTAIN DYES THAT ARE BOUND BY BOVINE SERUM ALBUMIN [J].

AINSWORT.S ;

FLANAGAN, MT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 194 (01) :213-&

[2]

Azzi A, 1974, Methods Enzymol, V32, P234

[3] ULTRAVIOLET FLUORESCENCE OF MUREX-TRUNCULUS HAEMOCYANIN IN RELATION TO BINDING OF COPPER AND OXYGEN [J].

BANNISTER, WH ;

WOOD, EJ .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1971, 40 (1B) :7-+

[4] FLUORESCENCE PROBES FOR STRUCTURE [J].

BRAND, L ;

GOHLKE, JR .

ANNUAL REVIEW OF BIOCHEMISTRY, 1972, 41 :843-+

[5]

BRAND L, 1967, METHOD ENZYMOL, V11, P776

[6] FLUORESCENCE AND LOCATION OF TRYPTOPHAN RESIDUES IN PROTEIN MOLECULES [J].

BURSTEIN, EA ;

VEDENKINA, NS ;

IVKOVA, MN .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1973, 18 (04) :263-279

[7]

CHIGNELL CF, 1972, METHOD PHARMACOL, V2, P33

[8] EPR STUDY OF NEUROSPORA TYROSINASE [J].

DEINUM, J ;

LERCH, K ;

REINHAMMAR, B .

FEBS LETTERS, 1976, 69 (01) :161-164

[9] EXPOSURE OF TRYPTOPHANYL RESIDUES IN PROTEINS - QUANTITATIVE-DETERMINATION BY FLUORESCENCE QUENCHING STUDIES [J].

EFTINK, MR ;

GHIRON, CA .

BIOCHEMISTRY, 1976, 15 (03) :672-680

[10] FLUORESCENCE QUENCHING STUDIES WITH PROTEINS [J].

EFTINK, MR ;

GHIRON, CA .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (02) :199-227

← 1 2 3 4 5 →