Extracellular vesicles (EVs), including the nano-sized exosomes, have the capacity to transfer multiple functional molecules between cells. In cell culture experiments, fetal bovine serum (FBS) is often used to supplement cell culture medium as a nutrient, but it is important to know that the FBS also contain significant quantities of EVs. The aim of the current study was to determine whether the FBS EVs can influence cultured cell phenotype, and secondly to determine the efficiency of FBS-EVelimination protocols. Firstly, FBS that had not been depleted of EVs induced a migratory phenotype in a lung cancer epithelial cell line (A549 cells), an effect that could be mimicked by isolated FBS EVs alone. FBS-derived EVs also contained RNA, which was protected from consecutive proteinase K and RNase A treatment. Comparison of common isolation protocols suggested that an 18-hour centrifugation period eliminates approximately 95% of RNA-containing FBS EVs, whereas a 1.5-hour protocol is insufficient. In conclusion, this study shows that FBS EVs substantially influence cultured cell behaviour, but also that they can be virtually removed by an 18-hour ultracentrifugation protocol.
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Arroyo, Jason D.
;
Chevillet, John R.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Chevillet, John R.
;
Kroh, Evan M.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Kroh, Evan M.
;
Ruf, Ingrid K.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Ruf, Ingrid K.
;
Pritchard, Colin C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Lab Med, Med Ctr, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Pritchard, Colin C.
;
Gibson, Donald F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Lab Med, Med Ctr, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Gibson, Donald F.
;
Mitchell, Patrick S.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Mitchell, Patrick S.
;
Bennett, Christopher F.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Univ Washington, Mol & Cellular Biol Program, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Bennett, Christopher F.
;
Pogosova-Agadjanyan, Era L.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Pogosova-Agadjanyan, Era L.
;
Stirewalt, Derek L.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Stirewalt, Derek L.
;
Tait, Jonathan F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Lab Med, Med Ctr, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Tait, Jonathan F.
;
Tewari, Muneesh
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Fred Hutchinson Canc Res Ctr, Dept Publ Hlth Sci, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
机构:
Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA
St Marys Coll, Dept Biol, Moraga, CA 94556 USAUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA
Berleman, James
;
Auer, Manfred
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USAUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Arroyo, Jason D.
;
Chevillet, John R.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Chevillet, John R.
;
Kroh, Evan M.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Kroh, Evan M.
;
Ruf, Ingrid K.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Ruf, Ingrid K.
;
Pritchard, Colin C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Lab Med, Med Ctr, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Pritchard, Colin C.
;
Gibson, Donald F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Lab Med, Med Ctr, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Gibson, Donald F.
;
Mitchell, Patrick S.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Mitchell, Patrick S.
;
Bennett, Christopher F.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Univ Washington, Mol & Cellular Biol Program, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Bennett, Christopher F.
;
Pogosova-Agadjanyan, Era L.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Pogosova-Agadjanyan, Era L.
;
Stirewalt, Derek L.
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Stirewalt, Derek L.
;
Tait, Jonathan F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Lab Med, Med Ctr, Seattle, WA 98195 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Tait, Jonathan F.
;
Tewari, Muneesh
论文数: 0引用数: 0
h-index: 0
机构:
Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
Fred Hutchinson Canc Res Ctr, Dept Publ Hlth Sci, Seattle, WA 98109 USAFred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA
机构:
Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA
St Marys Coll, Dept Biol, Moraga, CA 94556 USAUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA
Berleman, James
;
Auer, Manfred
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USAUniv Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA