THE DISTRIBUTION OF SINDBIS VIRUS PROTEINS IN MOSQUITO CELLS AS DETERMINED BY IMMUNOFLUORESCENCE AND IMMUNOELECTRON MICROSCOPY

被引：14

作者：

MILLER, ML

BROWN, DT

机构：

[1] UNIV TEXAS,CELL RES INST,AUSTIN,TX 78713

[2] UNIV TEXAS,DEPT MICROBIOL,AUSTIN,TX 78713

来源：

JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷

关键词：

D O I：

10.1099/0022-1317-74-2-293

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two Aedes albopictus (mosquito) subclones, C7-10 and C6/36, were examined by immunofluorescence and immunoelectron microscopy for the distribution of Sindbis virus structural and non-structural proteins. Both the viral glycoproteins, E1 and E2, and the non-structural proteins, nsP1 and nsP2, were found within vesicles and electron-dense, amorphous matrices associated with Sindbis virus infection. The labelling patterns indicated that both replication of viral RNA and production of virus particles were localized within the same structures in the infected cell. The data support previous reports that alphavirus infection is contained within specific structures in the cytoplasm and provide additional evidence that the C6/36 and C7-10 subclones may represent different tissue types in the adult insect.

引用

页码：293 / 298

页数：6

共 21 条

[1] ISOLATION AND CHARACTERIZATION OF CONDITIONAL-LETHAL MUTANTS OF SINDBIS VIRUS [J].

BURGE, BW ;

PFEFFERKORN, ER .

VIROLOGY, 1966, 30 (02) :204-+

[2] EXCLUSION OF SUPERINFECTING HOMOLOGOUS VIRUS BY SINDBIS VIRUS-INFECTED AEDES-ALBOPICTUS (MOSQUITO) CELLS [J].

CONDREAY, LD ;

BROWN, DT .

JOURNAL OF VIROLOGY, 1986, 58 (01) :81-86

[3] EFFECT OF ACTINOMYCIN-D AND CYCLOHEXIMIDE ON REPLICATION OF SINDBIS VIRUS IN AEDES-ALBOPICTUS (MOSQUITO) CELLS [J].

CONDREAY, LD ;

ADAMS, RH ;

EDWARDS, J ;

BROWN, DT .

JOURNAL OF VIROLOGY, 1988, 62 (08) :2629-2635

[4] SUPPRESSION OF RNA-SYNTHESIS BY A SPECIFIC ANTIVIRAL ACTIVITY IN SINDBIS VIRUS-INFECTED AEDES-ALBOPICTUS CELLS [J].

CONDREAY, LD ;

BROWN, DT .

JOURNAL OF VIROLOGY, 1988, 62 (01) :346-348

[5] AMINO ACID METABOLISM IN MAMMALIAN CELL CULTURES [J].

EAGLE, H .

SCIENCE, 1959, 130 (3373) :432-437

[6] REQUIREMENT OF CELL-NUCLEUS FOR SINDBIS VIRUS-REPLICATION IN CULTURED AEDES-ALBOPICTUS CELLS [J].

ERWIN, C ;

BROWN, DT .

JOURNAL OF VIROLOGY, 1983, 45 (02) :792-799

[7] MORPHOGENESIS OF SINDBIS VIRUS IN CULTURED AEDES-ALBOPICTUS CELLS [J].

GLIEDMAN, JB ;

SMITH, JF ;

BROWN, DT .

JOURNAL OF VIROLOGY, 1975, 16 (04) :913-926

[8] PROCESSING THE NONSTRUCTURAL POLYPROTEINS OF SINDBIS VIRUS - STUDY OF THE KINETICS INVIVO BY USING MONOSPECIFIC ANTIBODIES [J].

HARDY, WR ;

STRAUSS, JH .

JOURNAL OF VIROLOGY, 1988, 62 (03) :998-1007

[9] EVOLUTION AND PROPERTIES OF AEDES-ALBOPICTUS CELL-CULTURES PERSISTENTLY INFECTED WITH SINDBIS VIRUS [J].

IGARASHI, A ;

KOO, R ;

STOLLAR, V .

VIROLOGY, 1977, 82 (01) :69-83

[10] ISOLATION OF A SINGHS AEDES-ALBOPICTUS CELL CLONE SENSITIVE TO DENGUE AND CHIKUNGUNYA VIRUSES [J].

IGARASHI, A .

JOURNAL OF GENERAL VIROLOGY, 1978, 40 (SEP) :531-544

← 1 2 3 →