CAPILLARY ZONE ELECTROPHORESIS OF PROTEINS WITH A DYNAMIC SURFACTANT COATING - INFLUENCE OF A VOLTAGE GRADIENT ON THE SEPARATION EFFICIENCY

被引：58

作者：

MUIJSELAAR, WGHM

DEBRUIJN, CHMM

EVERAERTS, FM

机构：

[1] Laboratory of Instrumental Analysis, Eindhoven University of Technology, 5600 MB Eindhoven

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 605卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85035-R

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In capillary zone electrophoresis of proteins, the adsorption of the proteins on the capillary wall is a considerable problem that seriously impairs the separation efficiency. The use of a dynamic surfactant coating is a possible way to diminish this adsorption. Highly efficient separations were achieved with a cationic fluorinated buffer additive as a dynamic surfactant coating in untreated fused-silica capillaries at neutral pH. The influence of a voltage gradient on the separation efficiency is discussed and a simple relationship is presented to calculate effective mobilities under voltage gradient conditions.

引用

页码：115 / 123

页数：9

共 20 条

[1] DETERMINATION OF ABSOLUTE MOBILITIES, PK VALUES AND SEPARATION NUMBERS BY CAPILLARY ZONE ELECTROPHORESIS - EFFECTIVE MOBILITY AS A PARAMETER FOR SCREENING [J].