A RADIOIMMUNOASSAY FOR COLCHICINE USING A HIGHLY SPECIFIC, HIGH-AFFINITY MONOCLONAL-ANTIBODY

被引：5

作者：

ROUAN, SKE

OTTERNESS, IG

GANS, DJ

RHODES, CT

机构：

[1] UNIV RHODE ISL,DEPT PHARMACEUT,KINGSTON,RI 02881

[2] PFIZER INC,DIV CENT RES,DEPT IMMUNOL & INFECT DIS,GROTON,CT 06340

[3] PFIZER INC,DIV CENT RES,DEPT CLIN RES,GROTON,CT 06340

来源：

DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY | 1991年 / 17卷 / 17期

关键词：

D O I：

10.3109/03639049109048082

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A sensitive and selective radioimmunoassay has been developed using a colchicine binding monoclonal antibody. The assay procedure uses a charcoal suspension to separate antibody bound and free colchicine and can be performed in less than two hours. Using the high affinity antibody. as little as 0.3 ng/ml of colchicine in serum can be detected. These results provide the framework for a fully validated clinical assay for therapeutic monitoring of colchicine.

引用

页码：2391 / 2400

页数：10

共 11 条

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