VAUGHN AND LULAI - SUBERIN STAINS COMPARISON OF FLUORESCENT STAINS FOR THE DETECTION OF SUBERIN IN POTATO PERIDERM

被引：12

作者：

VAUGHN, SF ^{[1
]}

LULAI, EC ^{[1
]}

机构：

[1] USDA ARS,RED RIVER VALLEY POTATO RES LAB,E GRAND FORKS,MN 56721

来源：

AMERICAN POTATO JOURNAL | 1991年 / 68卷 / 10期

关键词：

ANILINE BLUE; BERBERINE; CRYSTAL VIOLET; LIGNIN;

D O I：

10.1007/BF02853742

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Several fluorescent staining procedures to detect suberin in normal periderm of potato were assessed. Among the stains tested, treatment with the alkaloid fluorochrome berberine followed by counterstaining with crystal violet appears to be the most sensitive combination. Crystal violet increased contrast by quenching lignin fluorescence which is also present in the periderm cell walls. This technique may be useful for studying the role of suberin in disease resistance and wound healing of potato tubers and other tissues.

引用

页码：667 / 674

页数：8

共 13 条

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