MICROBIOLOGICAL ASSAY FOR VITAMIN-B-12 PERFORMED IN 96-WELL MICROTITRE PLATES

被引：244

作者：

KELLEHER, BP ^{[1
]}

BROIN, SDO ^{[1
]}

机构：

[1] ST JAMES HOSP,DEPT HAEMATOL,JAMES ST,DUBLIN 8,IRELAND

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 44卷 / 07期

关键词：

D O I：

10.1136/jcp.44.7.592

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.

引用

页码：592 / 595

页数：4

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