NONRADIOACTIVE COLONY HYBRIDIZATION ASSAY FOR DETECTION AND ENUMERATION OF ENTEROLOXIGENIC CLOSTRIDIUM-PERFRINGENS IN RAW BEEF

被引：9
|
作者：
BAEZ, LA [1 ]
JUNEJA, VK [1 ]
机构：
[1] USDA ARS,EASTERN REG RES CTR,PHILADELPHIA,PA 19118
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 02期
关键词：
D O I：
10.1128/AEM.61.2.807-810.1995
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A DNA probe endolabeled with digoxigenin by PCR was developed to detect and enumerate enterotoxigenic Clostridium perfringens in raw beef. After 2 h of hybridization, membranes were developed by using an anti digoxigenin-alkaline phosphatase conjugated antibody. The resulting chromogenic reaction allowed us to detect and enumerate less than or equal to 10 CFU of C. perfringens per g.
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页码：807 / 810
页数：4
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