A PLASTIC EMBEDDING TECHNIQUE FOR ANALYZING FLUORESCENT DEXTRAN-AMINE LABELED NEURONAL PROFILES

被引：10

作者：

FRITZSCH, B ^{[1
]}

NORTHCUTT, RG ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT NEUROSCI,LA JOLLA,CA 92093

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1992年 / 67卷 / 03期

关键词：

FLUORESCENT DEXTRAN-AMINES; PLASTIC EMBEDDING;

D O I：

10.3109/10520299209110026

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A plastic embedding technique employing fluorescently labelled dextran-amines is described. After application of tracer to cut nerves and appropriate transport time, animals were fixed in paraformaldehyde. Subsequently their brains were dissected, heads and brains were dehydrated, embedded in methacrylate and sectioned serially on a rotary microtome. Plastic sections allow high resolution of single neuron profiles and complete serial reconstruction of undistorted sections, including embryos with large amounts of yolk. In conjunction with whole mount analysis and double labelling, this technique can accurately reveal the spatial relationships of nerve components throughout development.

引用

页码：153 / 157

页数：5

共 6 条

[1] SEQUENTIAL DOUBLE LABELING WITH DIFFERENT FLUORESCENT DYES COUPLED TO DEXTRAN AMINES AS A TOOL TO ESTIMATE THE ACCURACY OF TRACER APPLICATION AND OF REGENERATION [J].