A QUANTITATIVE-ANALYSIS OF THE ACCURACY OF INVIVO PH MEASUREMENTS WITH P-31 NMR-SPECTROSCOPY - ASSESSMENT OF PH MEASUREMENT METHODOLOGY

被引：37

作者：

MADDEN, A

LEACH, MO

SHARP, JC

COLLINS, DJ

EASTON, D

机构：

[1] INST CANC RES,JOINT DEPT PHYS,DOWNS RD,SUTTON SM2 5PT,SURREY,ENGLAND

[2] INST CANC RES,EPIDEMIOL SECT,SUTTON SM2 5PT,SURREY,ENGLAND

[3] ROYAL MARSDEN HOSP,JOINT DEPT PHYS,SUTTON SM2 5PT,SURREY,ENGLAND

[4] UNIV NOTTINGHAM,DEPT PHYS,NOTTINGHAM NG7 2RD,ENGLAND

来源：

NMR IN BIOMEDICINE | 1991年 / 4卷 / 01期

关键词：

D O I：

10.1002/nbm.1940040102

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

31P NMR spectroscopy provides a means of monitoring intracellular pH (pHi). In order to determine the significance of observed pH changes, an assessment of the measurement uncertainty is required. This paper considers the possible sources of error involved, firstly in chemical shift assignment and, secondly, in relating chemical shift to pH. A statistical analysis of these errors is presented and an expression for the accuracy of pH measurements obtained using in vivo 31P NMR spectroscopy is derived. Computer simulations and clinical measurements are considered to determine the likely error in clinical measurements. A comparison of pH measurement methodology using a centre‐of‐gravity calculation, Gaussian and Lorentzian fitting is also presented and discussed. Copyright © 1991 John Wiley & Sons, Ltd.

引用

页码：1 / 11

页数：11

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