ULTRASTRUCTURAL AND ELECTRON SPECTROSCOPIC ANALYSES OF CYANOBACTERIA AND BACTERIA

被引：26

作者：

GOLECKI, JR ^{[1
]}

HEINRICH, UR ^{[1
]}

机构：

[1] UNIV FREIBURG, INST BIOPHYS & STRAHLENBIOL, W-7800 FREIBURG, GERMANY

来源：

JOURNAL OF MICROSCOPY | 1991年 / 162卷

关键词：

CYANOBACTERIA; ELECTRON SPECTROSCOPIC IMAGING (ESI); ELECTRON ENERGY-LOSS SPECTROSCOPY (EELS); INCLUSION BODIES; NANOPLAST EMBEDDING; PHOSPHATE-ACCUMULATING BACTERIA; SEWAGE BACTERIA; SHEATH MATERIAL;

D O I：

10.1111/j.1365-2818.1991.tb03125.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The extracellular sheath material and some intracellular cell components of cyanobacteria and phosphate-accumulating sewage bacteria were analysed by electron spectroscopic imaging (ESI) and electron energy-loss spectroscopy (EELS). The specimens were embedded in water-soluble Nanoplast resin without any previous fixation and ultrathin sections were examined in a Zeiss CEM 902 microscope. A high sulphur content was detected in the inner sheath of the cyanobacterium Gloeothece. The elemental composition of some cell components and inclusion bodies, such as carboxysomes and cyanophycin, was determined by ESI and EELS. In addition, the phosphate content in specific granules of phosphate-accumulating sewage bacteria was estimated by EELS and nuclear magnetic resonance spectroscopy.

引用

页码：147 / 154

页数：8

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