CYTOCHROME-OXIDASE FROM THERMOPHILIC BACTERIUM PS3 CONTAINS A 4TH PROTEIN SUBUNIT

被引：7
作者：
GAI, WZ
SUN, SM
SONE, N
CHAN, SHP
机构：
[1] SYRACUSE UNIV, DEPT BIOL, SYRACUSE, NY 13244 USA
[2] JICHI MED SCH, DEPT BIOCHEM, MINAMI KAWACHI, TOCHIGI 32904, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)90347-P
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. "Western" blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III. © 1990.
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页码：414 / 421
页数：8
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