A YEAST-ESCHERICHIA-COLI SHUTTLE VECTOR CONTAINING THE M13 ORIGIN OF REPLICATION

被引：14
作者：
RHODES, N
COMPANY, M
ERREDE, B
机构：
[1] Department of Chemistry, University of North Carolina, Chapel Hill
来源：
PLASMID | 1990年 / 23卷 / 02期
关键词：
D O I：
10.1016/0147-619X(90)90036-C
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharyomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes. © 1990.
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页码：159 / 162
页数：4
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