CATALYTIC-OXIDATION OF NADH AT A METHYLENE-GREEN CHEMICALLY MODIFIED ELECTRODE AND FIA APPLICATIONS

被引：14

作者：

CHEN, HY ^{[1
]}

YU, AM ^{[1
]}

HAN, JL ^{[1
]}

MI, YZ ^{[1
]}

机构：

[1] NANJING UNIV,INST COORDINAT CHEM,NANJING 210093,PEOPLES R CHINA

来源：

ANALYTICAL LETTERS | 1995年 / 28卷 / 09期

基金：

中国国家自然科学基金;

关键词：

METHYLENE GREEN; MODIFIED ELECTRODES; NADH; ELECTROCATALYTIC OXIDATION; FLOW INJECTION ANALYSIS; AMPEROMETRIC DETECTION;

D O I：

10.1080/00032719508002766

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Chemically modified electrodes with Methylene Green (MG) incorporated into carbon paste exhibit excellent electrocatalytic ability for reduced nicotinamide adenine dinucleotide (NADH), reducing oxidation overpotential by 600mV compared with the direct oxidation of NADH at unmodified electrode. In flow injection analysis (FIA) of NADH, MG-modified carbon paste electrodes show high catalytic activity and stability, with linear response range over about 3 orders of magnitude and a detection limit of 1.0 pmol. Furthermore, the influence of various experimental conditions was explored for optimum analytical performance.

引用

页码：1579 / 1591

页数：13

共 17 条

[1] AN AMPEROMETRIC GLUCOSE ELECTRODE BASED ON CARBON PASTE, CHEMICALLY MODIFIED WITH GLUCOSE-DEHYDROGENASE, NICOTINAMIDE ADENINE-DINUCLEOTIDE, AND A PHENOXAZINE MEDIATOR, COATED WITH A POLY(ESTER SULFONIC-ACID) CATION EXCHANGER [J].

BREMLE, G ;

PERSSON, B ;

GORTON, L .

ELECTROANALYSIS, 1991, 3 (02) :77-86

[2] REGENERATION OF NICOTINAMIDE COFACTORS FOR USE IN ORGANIC-SYNTHESIS [J].

CHENAULT, HK ;

WHITESIDES, GM .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1987, 14 (02) :147-197

[3] CHEMICALLY MODIFIED ELECTRODES FOR THE ELECTROCATALYTIC OXIDATION OF NICOTINAMIDE COENZYMES [J].

GORTON, L .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1986, 82 :1245-1258

[4] AMPEROMETRIC GLUCOSE SENSORS BASED ON IMMOBILIZED GLUCOSE-OXIDIZING ENZYMES AND CHEMICALLY MODIFIED ELECTRODES [J].

GORTON, L ;

BREMLE, G ;

CSOREGI, E ;

JONSSONPETTERSSON, G ;

PERSSON, B .

ANALYTICA CHIMICA ACTA, 1991, 249 (01) :43-54

[5] CHEMICAL CROSS-LINKING OF A REDOX MEDIATOR THIONIN FOR ELECTROCATALYTIC OXIDATION OF REDUCED BETA-NICOTINAMIDE ADENINE-DINUCLEOTIDE [J].

HAJIZADEH, K ;

TANG, HT ;

HALSALL, HB ;

HEINEMAN, WR .

ANALYTICAL LETTERS, 1991, 24 (08) :1453-1469

[6]

HAN JH, IN PRESS SINICA CHIM

[7] ADSORPTION AND ELECTROCHEMICAL OXIDATION BEHAVIOR OF NADH AT A CLEAN PLATINUM-ELECTRODE [J].

JAEGFELDT, H .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1980, 110 (1-3) :295-302

[8]

KAKURA S, 1992, BIOELECTROCH BIOENER, V28, P387

[9] AMPEROMETRIC AND COULOMETRIC DETECTORS FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KISSINGER, PT .

ANALYTICAL CHEMISTRY, 1977, 49 (04) :A447-&

[10]

Michaelis L, 1913, BIOCHEM Z, V49, P333

← 1 2 →