THE ABILITY OF MEMBRANE-POTENTIAL DYES AND CALCAFLUOR WHITE TO DISTINGUISH BETWEEN VIABLE AND NONVIABLE BACTERIA

被引：96

作者：

MASON, DJ ^{[1
]}

LOPEZAMOROS, R ^{[1
]}

ALLMAN, R ^{[1
]}

STARK, JM ^{[1
]}

LLOYD, D ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF, SCH PURE & APPL BIOL, CARDIFF CF1 3TL, S GLAM, WALES

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 78卷 / 03期

关键词：

D O I：

10.1111/j.1365-2672.1995.tb05031.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.

引用

页码：309 / 315

页数：7

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