THE ABILITY OF MEMBRANE-POTENTIAL DYES AND CALCAFLUOR WHITE TO DISTINGUISH BETWEEN VIABLE AND NONVIABLE BACTERIA

被引：96

作者：

MASON, DJ ^{[1
]}

LOPEZAMOROS, R ^{[1
]}

ALLMAN, R ^{[1
]}

STARK, JM ^{[1
]}

LLOYD, D ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF, SCH PURE & APPL BIOL, CARDIFF CF1 3TL, S GLAM, WALES

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 78卷 / 03期

关键词：

D O I：

10.1111/j.1365-2672.1995.tb05031.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.

引用

页码：309 / 315

页数：7

共 27 条

[1] A RAPID ANALYTICAL TECHNIQUE FOR FLOW CYTOMETRIC ANALYSIS OF CELL VIABILITY USING CALCOFLUOR WHITE M2R [J].

BERGLUND, DL ;

TAFFS, RE ;

ROBERTSON, NP .

CYTOMETRY, 1987, 8 (04) :421-426

[2] COMPARATIVE MEASUREMENTS OF MEMBRANE-POTENTIALS WITH MICROELECTRODES AND VOLTAGE-SENSITIVE DYES [J].

BRAUNER, T ;

HULSER, DF ;

STRASSER, RJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 771 (02) :208-216

[3]

Carter Elizabeth A., 1993, P111

[4] ABSORPTION AND TRANSPORT OF FLUORESCENT BRIGHTENERS BY MICROORGANISMS [J].

DARKEN, MA .

APPLIED MICROBIOLOGY, 1962, 10 (05) :387-+

[5]

Davey Hazel M., 1993, P83

[6]

DIAPER JP, 1992, APPL MICROBIOL BIOT, V22, P1

[7]

EDDY AA, 1989, METHOD ENZYMOL, V172, P95

[8] SIMULTANEOUS IMAGING OF CELL AND MITOCHONDRIAL-MEMBRANE POTENTIALS [J].

FARKAS, DL ;

WEI, MD ;

FEBBRORIELLO, P ;

CARSON, JH ;

LOEW, LM .

BIOPHYSICAL JOURNAL, 1989, 56 (06) :1053-1069

[9] DETERMINATION OF THE MEMBRANE-POTENTIAL OF CULTURED MAMMALIAN SCHWANN-CELLS AND ITS SENSITIVITY TO POTASSIUM USING A THIOCARBOCYANINE FLUORESCENT DYE [J].

HARGITTAI, PT ;

YOUMANS, SJ ;

LIEBERMAN, EM .

GLIA, 1991, 4 (06) :611-616

[10] RAPID ASSESSMENT OF BACTERIAL VIABILITY AND VITALITY BY RHODAMINE 123 AND FLOW-CYTOMETRY [J].

KAPRELYANTS, AS ;

KELL, DB .

JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (05) :410-422

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