LOW HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) DNA BURDEN AS A MAJOR CAUSE FOR FAILURE TO DETECT HIV-1 DNA IN CLINICAL SPECIMENS BY PCR

被引：19

作者：

ZAZZI, M ^{[1
]}

ROMANO, L ^{[1
]}

CATUCCI, M ^{[1
]}

DEMILITO, A ^{[1
]}

ALMI, P ^{[1
]}

GONNELLI, A ^{[1
]}

RUBINO, M ^{[1
]}

VALENSIN, PE ^{[1
]}

机构：

[1] DIV INFECT DIS,SIENA,ITALY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 01期

关键词：

D O I：

10.1128/JCM.33.1.205-208.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To determine the sensitivity of a nested PCR procedure for detecting human immunodeficiency virus type 1 DNA in clinical specimens, 553 peripheral blood mononuclear cell samples obtained from 268 human immunodeficiency virus type 1-seropositive subjects were assayed by use of two independent primer sets for each sample. Overall, 1,088 of 1,106 (98.37%) reactions were positive. Investigation of the negative reactions showed that a low viral burden in some infected subjects, rather than primer-template mismatches, was the primary cause for the false-negative PCR results.

引用

页码：205 / 208

页数：4

共 45 条

[41] QUANTITATION OF HUMAN-IMMUNODEFICIENCY-VIRUS PROVIRUS AND CIRCULATING VIRUS - RELATIONSHIP WITH IMMUNOLOGICAL PARAMETERS [J].