LOW HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) DNA BURDEN AS A MAJOR CAUSE FOR FAILURE TO DETECT HIV-1 DNA IN CLINICAL SPECIMENS BY PCR

被引：19

作者：

ZAZZI, M ^{[1
]}

ROMANO, L ^{[1
]}

CATUCCI, M ^{[1
]}

DEMILITO, A ^{[1
]}

ALMI, P ^{[1
]}

GONNELLI, A ^{[1
]}

RUBINO, M ^{[1
]}

VALENSIN, PE ^{[1
]}

机构：

[1] DIV INFECT DIS,SIENA,ITALY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 01期

关键词：

D O I：

10.1128/JCM.33.1.205-208.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To determine the sensitivity of a nested PCR procedure for detecting human immunodeficiency virus type 1 DNA in clinical specimens, 553 peripheral blood mononuclear cell samples obtained from 268 human immunodeficiency virus type 1-seropositive subjects were assayed by use of two independent primer sets for each sample. Overall, 1,088 of 1,106 (98.37%) reactions were positive. Investigation of the negative reactions showed that a low viral burden in some infected subjects, rather than primer-template mismatches, was the primary cause for the false-negative PCR results.

引用

收藏

页码：205 / 208

页数：4

相关论文

共 45 条

[1] SIMPLE, SENSITIVE, AND SPECIFIC DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN CLINICAL SPECIMENS BY POLYMERASE CHAIN-REACTION WITH NESTED PRIMERS [J].

ALBERT, J ;

FENYO, EM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (07) :1560-1564

[2] DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TRANSCRIPTS IN PERIPHERAL-BLOOD LYMPHOCYTES BY THE POLYMERASE CHAIN-REACTION [J].

BAGNARELLI, P ;

MENZO, S ;

MANZIN, A ;

VARALDO, PE ;

MONTRONI, M ;

GIACCA, M ;

CLEMENTI, M .

JOURNAL OF VIROLOGICAL METHODS, 1991, 32 (01) :31-39

[3] VARIABLE RELATIONSHIP BETWEEN PROVIRAL DNA LOAD AND INFECTIOUS VIRUS TITER IN THE PERIPHERAL-BLOOD MONONUCLEAR-CELLS OF HIV-1-INFECTED INDIVIDUALS [J].

BIENIASZ, PD ;

ARIYOSHI, K ;

BOURELLY, MAD ;

BLOOR, S ;

FOXALL, RB ;

HARWOOD, EC ;

WEBER, JN .

AIDS, 1993, 7 (06) :803-806

[4] DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION IN YOUNG PEDIATRIC-PATIENTS BY USING POLYMERASE CHAIN-REACTION AND BIOTINYLATED PROBES [J].

BRANDT, CD ;

RAKUSAN, TA ;

SISON, AV ;

JOSEPHS, SH ;

SAXENA, ES ;

HERZOG, KD ;

PARROTT, RH ;

SEVER, JL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (01) :36-40

[5] FEW INFECTED CD4+ T-CELLS BUT A HIGH PROPORTION OF REPLICATION-COMPETENT PROVIRUS COPIES IN ASYMPTOMATIC HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION [J].

BRINCHMANN, JE ;

ALBERT, J ;

VARTDAL, F .

JOURNAL OF VIROLOGY, 1991, 65 (04) :2019-2023

[6] USE OF DRIED BLOOD SPOT SPECIMENS IN THE DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 BY THE POLYMERASE CHAIN-REACTION [J].

CASSOL, S ;

SALAS, T ;

ARELLA, M ;

NEUMANN, P ;

SCHECHTER, MT ;

OSHAUGHNESSY, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (04) :667-671

[7]

CLARK AGB, 1992, J ACQ IMMUN DEF SYND, V5, P52

[8] COAMPLIFIED POSITIVE CONTROL DETECTS INHIBITION OF POLYMERASE CHAIN-REACTIONS [J].

CONE, RW ;

HOBSON, AC ;

HUANG, MLW .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (12) :3185-3189

[9]

CONWAY B, 1990, J ACQ IMMUN DEF SYND, V3, P1059

[10] ENZYME-IMMUNOASSAY FOR DETECTION OF HYBRIDS BETWEEN PCR-AMPLIFIED HIV-1 DNA AND A RNA PROBE - PCR-EIA [J].

COUTLEE, F ;

YANG, BZ ;

BOBO, L ;

MAYUR, K ;

YOLKEN, R ;

VISCIDI, R .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1990, 6 (06) :775-784

← 1 2 3 4 5 →