DEHYDROGENASE-MODIFIED CARBON-FIBER MICROELECTRODES FOR THE MEASUREMENT OF NEUROTRANSMITTER DYNAMICS .2. COVALENT MODIFICATION UTILIZING AVIDIN BIOTIN TECHNOLOGY

被引：120

作者：

PANTANO, P ^{[1
]}

KUHR, WG ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT CHEM,RIVERSIDE,CA 92521

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 05期

关键词：

D O I：

10.1021/ac00053a023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Dehydrogenase enzymes are immobilized onto carbon-fiber microelectrode surfaces via avidin-biotin technology and a covalently linked hydrophilic tether. The avidin-biotin coupling strategy allows the selectivity of the electrochemical measurement to be easily changed without reoptimizing the immobilization conditions. Optimized derivatization conditions are demonstrated for dimer, tetramer, and hexamer dehydrogenases. Nonelectroactive substrates (ethanol, glucose-6-phosphate, and glutamate) are quantitated through the detection of enzyme-generated NADH by fast scan cyclic staircase voltammetry (100 V/s). The glutamate dehydrogenase-modified microelectrode possesses a 300-ms response time with a detection limit of 0.5 mM glutamate and a 1-60 mM linear concentration range.

引用

页码：623 / 630

页数：8

共 34 条

[1] INTRACELLULAR ANALYSIS WITH AN IMMOBILIZED-ENZYME GLUCOSE ELECTRODE HAVING A 2-MU-M DIAMETER AND SUBSECOND RESPONSE-TIMES [J].