DNA/RNA Detection Using DNA-Templated Few-Atom Silver Nanoclusters

被引：70

作者：

Obliosca, Judy M. ^{[1
]}

Liu, Cong ^{[1
]}

Batson, Robert Austin ^{[1
]}

Babin, Mark C. ^{[2
]}

Werner, James H. ^{[3
]}

Yeh, Hsin-Chih ^{[1
]}

机构：

[1] Univ Texas Austin, Cockrell Sch Engn, Dept Biomed Engn, Austin, TX 78712 USA

[2] Univ Texas Austin, Coll Nat Sci, Austin, TX 78712 USA

[3] Los Alamos Natl Lab, Ctr Integrated Nanotechnol, Los Alamos, NM 78745 USA

来源：

BIOSENSORS-BASEL | 2013年 / 3卷 / 02期

关键词：

DNA/RNA detection; few-atom silver nanoclusters; single-nucleotide polymorphism detection; turn-on sensors;

D O I：

10.3390/bios3020185

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA-templated few-atom silver nanoclusters (DNA/Ag NCs) are a new class of organic/inorganic composite nanomaterials whose fluorescence emission can be tuned throughout the visible and near-IR range by simply programming the template sequences. Compared to organic dyes, DNA/Ag NCs can be brighter and more photostable. Compared to quantum dots, DNA/Ag NCs are smaller, less prone to blinking on long timescales, and do not have a toxic core. The preparation of DNA/Ag NCs is simple and there is no need to remove excess precursors as these precursors are non-fluorescent. Our recent discovery of the fluorogenic and color switching properties of DNA/Ag NCs have led to the invention of new molecular probes, termed NanoCluster Beacons (NCBs), for DNA detection, with the capability to differentiate single-nucleotide polymorphisms by emission colors. NCBs are inexpensive, easy to prepare, and compatible with commercial DNA synthesizers. Many other groups have also explored and taken advantage of the environment sensitivities of DNA/Ag NCs in creating new tools for DNA/RNA detection and single-nucleotide polymorphism identification. In this review, we summarize the recent trends in the use of DNA/Ag NCs for developing DNA/RNA sensors.

引用

页码：185 / 200

页数：16