FLUORESCENCE STUDY OF PRECRYSTALLIZATION OF RIBONUCLEASE-A - EFFECT OF SALTS

被引：16

作者：

CROSIO, MP

JULLIEN, M

机构：

[1] Laboratoire de Biologie Physicochimique, Université Paris-Sud, F-91405 Orsay

来源：

JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期

关键词：

D O I：

10.1016/0022-0248(92)90227-A

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The effects of different salts on protein-protein interactions in concentrated ribonuclease A solutions have been investigated using fluorescence anisotropy. It is observed that fluorescence anisotropy always exhibits a linear dependence on protein concentration. The virial coefficient remains constant for salt concentrations which do not lead to crystallization whereas it sharply increases for salt concentrations corresponding to crystal growth. This peculiar behavior has permitted us to determine new crystallization conditions.

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页码：66 / 70

页数：5

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