STRUCTURAL FEATURES OF THE TRANSACTIVATION RESPONSE RNA ELEMENT OF EQUINE INFECTIOUS-ANEMIA VIRUS

被引:24
|
作者
HOFFMAN, DW
COLVIN, RA
GARCIABLANCO, MA
WHITE, SW
机构
[1] DUKE UNIV,MED CTR,DEPT MICROBIOL,CELL GROWTH REGULAT & ONCOGENESIS SECT,DURHAM,NC 27710
[2] DUKE UNIV,MED CTR,DEPT MED,DURHAM,NC 27710
关键词
D O I
10.1021/bi00055a015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 25-nucleotide RNA with the sequence of the trans-activation response (TAR) element of equine infectious anemia virus (EIAV) was analyzed by biochemical methods and by one- and two-dimensional NMR spectroscopy. NMR, nuclease probing, and polyacrylamide gel migration rates show that the RNA consists of an A-helical stem capped by two non-Watson-Crick U-G base pairs and a compact four-nucleotide loop. The loop is stabilized by base stacking, with loop nucleotides C12 and C15 stacked upon U11 and G16, respectively. Near the 5' end of the molecule, the stem contains a bulge at nucleotide C2, most likely a result of base pairing between G1 and C25. A method for distinguishing RNA stem-loops from palindromic dimers is described and was used to confirm that the EIAV TAR RNA has a stem-loop structure at conditions used for NMR spectroscopy. RNase A and RNase T1 cleavage patterns are consistent with the structural features derived from the NMR data.
引用
收藏
页码:1096 / 1104
页数:9
相关论文
共 50 条
  • [21] CHARACTERIZATION OF THE INFECTION OF EQUINE FIBROBLASTS BY EQUINE INFECTIOUS-ANEMIA VIRUS
    KLEVJERANDERSON, P
    CHEEVERS, WP
    CRAWFORD, TB
    ARCHIVES OF VIROLOGY, 1979, 60 (3-4) : 279 - 289
  • [22] MUTATIONAL ANALYSIS OF THE EQUINE INFECTIOUS-ANEMIA VIRUS TAT-RESPONSIVE ELEMENT
    CARVALHO, M
    DERSE, D
    JOURNAL OF VIROLOGY, 1991, 65 (07) : 3468 - 3474
  • [23] CHARACTERIZATION OF INFECTIOUS MOLECULAR CLONES OF EQUINE INFECTIOUS-ANEMIA VIRUS
    PAYNE, SL
    RAUSCH, J
    RUSHLOW, K
    MONTELARO, RC
    ISSEL, C
    FLAHERTY, M
    PERRY, S
    SELLON, D
    FULLER, F
    JOURNAL OF GENERAL VIROLOGY, 1994, 75 : 425 - 429
  • [24] STUDIES ON EQUINE INFECTIOUS-ANEMIA VIRUS TRANSMISSION BY INSECTS
    ISSEL, CJ
    FOIL, LD
    JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 1984, 184 (03) : 293 - 297
  • [25] SEROLOGIC SURVEY FOR EQUINE INFECTIOUS-ANEMIA VIRUS IN LOUISIANA
    ISSEL, CJ
    ADAMS, WV
    JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 1979, 174 (03): : 286 - 288
  • [26] HEMAGGLUTINATION BY SEVERAL STRAINS OF EQUINE INFECTIOUS-ANEMIA VIRUS
    SENTSUI, H
    KONO, Y
    ARCHIVES OF VIROLOGY, 1981, 67 (01) : 75 - 84
  • [27] INACTIVATION OF EQUINE INFECTIOUS-ANEMIA VIRUS BY CHEMICAL DISINFECTANTS
    SHEN, DT
    CRAWFORD, TB
    GORHAM, JR
    MCGUIRE, TC
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1977, 38 (08) : 1217 - 1219
  • [28] PATTERN OF TRANSCRIPTION OF THE GENOME OF EQUINE INFECTIOUS-ANEMIA VIRUS
    NOIMAN, S
    YANIV, A
    SHERMAN, L
    TRONICK, SR
    GAZIT, A
    JOURNAL OF VIROLOGY, 1990, 64 (04) : 1839 - 1843
  • [29] INVITRO HOST RANGE OF EQUINE INFECTIOUS-ANEMIA VIRUS
    BENTON, CV
    BROWN, BL
    HARSHMAN, JS
    GILDEN, RV
    INTERVIROLOGY, 1981, 16 (04) : 225 - 232