ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE

被引：973

作者：

BARBAS, CF

KANG, AS

LERNER, RA

BENKOVIC, SJ

机构：

[1] SCRIPPS RES INST, DEPT CHEM, LA JOLLA, CA 92037 USA

[2] PENN STATE UNIV, DEPT CHEM, UNIVERSITY PK, PA 16802 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 18期

关键词：

COMBINATORIAL IMMUNOGLOBULIN REPERTOIRE; FILAMENTOUS PHAGE; SURFACE EXPRESSION; HUMAN ANTIBODIES; CATALYTIC ANTIBODIES;

D O I：

10.1073/pnas.88.18.7978

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

引用

页码：7978 / 7982

页数：5

共 24 条

[1] HORMONE PHAGE - AN ENRICHMENT METHOD FOR VARIANT PROTEINS WITH ALTERED BINDING-PROPERTIES
BASS, S
GREENE, R
WELLS, JA
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1990, 8 (04): : 309 - 314
[2] EFFECTS OF BACTERIOPHAGE-F1 GENE-III PROTEIN ON THE HOST-CELL MEMBRANE
BOEKE, JD
MODEL, P
ZINDER, ND
[J]. MOLECULAR & GENERAL GENETICS, 1982, 186 (02): : 185 - 192
[3] HUMAN AND MOUSE MONOCLONAL-ANTIBODIES BY REPERTOIRE CLONING
BURTON, DR
[J]. TRENDS IN BIOTECHNOLOGY, 1991, 9 (05) : 169 - 175
[4] INFLUENZA-VIRUS HEMAGGLUTININ-SPECIFIC ANTIBODIES ISOLATED FROM A COMBINATORIAL EXPRESSION LIBRARY ARE CLOSELY RELATED TO THE IMMUNE-RESPONSE OF THE DONOR
CATON, AJ
KOPROWSKI, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) : 6450 - 6454
[5] MEMBRANE BIOGENESIS - COTRANSLATIONAL INTEGRATION OF THE BACTERIOPHAGE-F1 COAT PROTEIN INTO AN ESCHERICHIA-COLI MEMBRANE-FRACTION
CHANG, CN
MODEL, P
BLOBEL, G
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (03) : 1251 - 1255
[6] GENE-III PROTEIN OF FILAMENTOUS PHAGES - EVIDENCE FOR A CARBOXYL-TERMINAL DOMAIN WITH A ROLE IN MORPHOGENESIS
CRISSMAN, JW
SMITH, GP
[J]. VIROLOGY, 1984, 132 (02) : 445 - 455
[7] PEPTIDES ON PHAGE - A VAST LIBRARY OF PEPTIDES FOR IDENTIFYING LIGANDS
CWIRLA, SE
PETERS, EA
BARRETT, RW
DOWER, WJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) : 6378 - 6382
[8] RANDOM PEPTIDE LIBRARIES - A SOURCE OF SPECIFIC PROTEIN-BINDING MOLECULES
DEVLIN, JJ
PANGANIBAN, LC
DEVLIN, PE
[J]. SCIENCE, 1990, 249 (4967) : 404 - 406
[9] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS
ELLINGTON, AD
SZOSTAK, JW
[J]. NATURE, 1990, 346 (6287) : 818 - 822
[10] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS
HANAHAN, D
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) : 557 - 580

← 1 2 3 →