ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE

被引：978
作者：
BARBAS, CF
KANG, AS
LERNER, RA
BENKOVIC, SJ
机构：
[1] SCRIPPS RES INST, DEPT CHEM, LA JOLLA, CA 92037 USA
[2] PENN STATE UNIV, DEPT CHEM, UNIVERSITY PK, PA 16802 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 18期
关键词：
COMBINATORIAL IMMUNOGLOBULIN REPERTOIRE; FILAMENTOUS PHAGE; SURFACE EXPRESSION; HUMAN ANTIBODIES; CATALYTIC ANTIBODIES;
D O I：
10.1073/pnas.88.18.7978
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.
引用
收藏
页码：7978 / 7982
页数：5
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