ONLINE INTERNAL SURFACE REVERSED-PHASE CLEANING - THE DIRECT HPLC ANALYSIS OF CRUDE BIOLOGICAL SAMPLES - APPLICATION TO THE KINETICS OF DEGRADATION OF OLIGONUCLEOTIDES IN CELL-CULTURE MEDIUM

被引：33
作者：
POMPON, A
LEFEBVRE, I
IMBACH, JL
机构：
[1] Laboratoire de Chimie Bio-Organique, U.R.A. CNRS No. 488, Université Montpellier II, 34095 Montpellier cedex 5, Case 008, Place E. Bataillon
来源：
BIOCHEMICAL PHARMACOLOGY | 1992年 / 43卷 / 08期
关键词：
D O I：
10.1016/0006-2952(92)90709-R
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
An "on-line" HPLC analysis of crude biological samples is described. A precolumn of internal surface reversed-phase material allows the passage of proteins and other unwanted products while retaining analyses which are transferred, concentrated and chromatographed on a conventional reverse-phase or ion-exchange HPLC column. This protocol allows precise kinetics of the degradation of an oligonucleotide in cell culture to be obtained without radiolabeling or sample preparation,
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页码：1769 / 1775
页数：7
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