DEVELOPMENT AND EVALUATION OF AN ENZYME-IMMUNOASSAY FOR SPORIDESMIN IN PASTURE

被引：0
|
作者：
GOLLIN, RG
BRIGGS, LR
TOWERS, NR
机构：
来源：
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH | 1995年 / 38卷 / 03期
关键词：
ELISA; ENZYME IMMUNOASSAY; PASTURE; PITHOMYCES CHARTARUM; SPORIDESMIN;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
An enzyme immunoassay was developed which could detect sporidesmin in pasture samples. The assay had a linear working range of 0.4-40 ng/ml sporidesmin A while validation using pasture extracts gave mean percentage recoveries of 124% for samples containing 1 ng/ml sporidesmin and 97% for extracts containing 20 ng/ml sporidesmin. The sporidesmin levels and numbers of Pithomyces chartarum spores from many pasture samples were compared. The amount of sporidesmin detected generally corresponded to the number of spores found in the sample.
引用
收藏
页码：297 / 302
页数：6
相关论文
共 50 条
  • [21] DEVELOPMENT OF AN ENZYME-IMMUNOASSAY FOR RAT SOLUBLE INTERLEUKIN-2 RECEPTORS
    POCKLEY, AG
    WILLIAMS, S
    REID, SD
    BOWLES, MJ
    JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 182 (01) : 81 - 84
  • [22] DEVELOPMENT OF AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF TOBACCO ALKALOIDS IN PLANT-MATERIAL
    FLINIAUX, MA
    MANCEAU, F
    JACQUINDUBREUIL, A
    PHYTOCHEMICAL ANALYSIS, 1992, 3 (05) : 223 - 226
  • [23] Development and evaluation of enzyme immunoassay for bromperidol
    Someya, T
    Shibasaki, M
    Takahashi, S
    Shirai, A
    Funaoka, H
    Sunahara, N
    JAPANESE JOURNAL OF NEUROPSYCHOPHARMACOLOGY, 1996, 18 (01): : 29 - 36
  • [24] NOVEL AND SENSITIVE NONCOMPETITIVE ENZYME-IMMUNOASSAY (HETERO-2-SITE ENZYME-IMMUNOASSAY) FOR ARGININE VASOPRESSIN IN PLASMA
    HASHIDA, S
    TANAKA, K
    YAMAMOTO, N
    UNO, T
    YAMAGUCHI, K
    ISHIKAWA, E
    ANALYTICAL LETTERS, 1991, 24 (07) : 1109 - 1123
  • [25] DETERMINATION OF WEATHERED ATRAZINE RESIDUES IN SOIL BY ENZYME-IMMUNOASSAY AND HPLC - AN EVALUATION STUDY
    SCHEWES, R
    WUST, S
    LEPSCHYVONGLEISSENTHALL, J
    MAIDL, FX
    SUSS, A
    HOCK, B
    FISCHBECK, G
    ANALYTICAL LETTERS, 1994, 27 (03) : 487 - 494
  • [26] DIAGNOSIS OF UROGENITAL GONORRHEA - EVALUATION OF AN ENZYME-IMMUNOASSAY AND USE OF URINE AS A NONINVASIVE SPECIMEN
    WONG, KC
    HO, BSW
    EGGLESTONE, SI
    LEWIS, WHP
    BRITISH JOURNAL OF BIOMEDICAL SCIENCE, 1994, 51 (04) : 312 - 315
  • [27] DEVELOPMENT OF ULTRASENSITIVE ENZYME-IMMUNOASSAY REVIEWED WITH EMPHASIS ON FACTORS WHICH LIMIT THE SENSITIVITY
    ISHIKAWA, E
    HASHIDA, S
    KOHNO, T
    MOLECULAR AND CELLULAR PROBES, 1991, 5 (02) : 81 - 95
  • [28] RAT PANCREATIC PHOSPHOLIPASE-A2 - PURIFICATION, LOCALIZATION, AND DEVELOPMENT OF AN ENZYME-IMMUNOASSAY
    KORTESUO, PT
    HIETARANTA, AJ
    JAMIA, M
    HIRSIMAKI, P
    NEVALAINEN, TJ
    INTERNATIONAL JOURNAL OF PANCREATOLOGY, 1993, 13 (02) : 111 - 118
  • [29] RAPID AND SPECIFIC ENZYME-IMMUNOASSAY FOR MAKISTERONE-A
    ROYER, C
    PORCHERON, P
    PRADELLES, P
    KERHOAS, L
    EINHORN, J
    MAUCHAMP, B
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1995, 25 (02) : 235 - 240
  • [30] ENZYME-IMMUNOASSAY FOR THE DETECTION OF LEVAMISOLE IN MEAT AND MILK
    SILVERLIGHT, JJ
    JACKMAN, R
    ANALYST, 1994, 119 (12) : 2705 - 2706
12345