ENZYMATIC-SYNTHESIS OF TRITIUM-LABELED PROSTAGLANDIN D-2 AND ITS CONVERSION TO OTHER PROSTAGLANDINS

被引：7
|
作者：
SHRAM, SI
LAZURKINA, TY
SHEVCHENKO, VP
NAGAEV, IY
MYASOEDOV, NF
机构：
[1] Laboratory of Isotopically Labeled Physiologically Active Compounds, Institute of Molecular Genetics Kurchatov Square, Moscow
来源：
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 1994年 / 34卷 / 04期
关键词：
TRITIUM LABELED PROSTAGLANDIN D-2; ENZYMATIC SYNTHESIS; H-3] PGD(2) DERIVATIVES; L-SELECTRIDE;
D O I：
10.1002/jlcr.2580340407
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The one-stage enzymatic synthesis of tritium-labelled prostaglandin D-2 from labelled arachidonic acid was performed by using the enzyme system PGH-synthetase/PGH-PGD-isomerase. By enzymatic and chemical transformation of [H-3]PGD(2) the following compounds were obtained: 15-keto-13, 14-dihydro-[H-3]PGD(2), 9 alpha,11 beta-[H-3]PGF(2), 9-deoxy-Delta(9)-[H-3]-PGD(2) ([H-3]PGJ(2)) and Delta(12)-13, 14-dihydro-[H-3]PGJ(2). It was found that L-selectride is a more effective reducing agent than sodium borohydride in the synthesis of 9 alpha,11 beta-[H-3]PGF(2).
引用
收藏
页码：359 / 366
页数：8
相关论文
共 50 条
  • [31] SYNTHESIS OF TRITIUM-LABELED 2-VINYL DIHYDROSPHINGOSINE-1-PHOSPHATE
    BOUMENDJEL, A
    MILLER, SPF
    JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 1995, 36 (04): : 377 - 383
  • [32] SYNTHESIS OF TRITIUM-LABELED 2-VINYL DIHYDROSPHINGOSINE-1-PHOSPHATE
    BOUMENDJEL, A
    MILLER, SPF
    JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 1995, 36 (03): : 243 - 249
  • [33] TRITIUM-LABELED COMPOUNDS .12. NOTE ON SYNTHESIS OF D-GLUCOSE-2-T AND D-MANNOSE-2-T
    ISBELL, HS
    FRUSH, HL
    WADE, CWR
    FATIADI, AJ
    JOURNAL OF RESEARCH OF THE NATIONAL BUREAU OF STANDARDS SECTION A-PHYSICS AND CHEMISTRY, 1969, A 73 (01): : 75 - +
  • [34] SYNTHESIS OF HIGH SPECIFIC ACTIVITY TRITIUM-LABELED CHLOROETHYLCYCLOHEXYLNITROSOUREA AND ITS APPLICATION TO THE STUDY OF DNA MODIFICATION
    SIEW, EL
    HABRAKEN, Y
    LUDLUM, DB
    JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 1991, 29 (02): : 223 - 230
  • [35] SYNTHESIS OF TRITIUM-LABELED PROSTAGLANDIN E-1 AND ITS USE AS A RADIOLIGAND IN STUDYING PGE1 BINDING TO HUMAN-PLATELETS
    SHEVCHENKO, VP
    NAGAYEV, IY
    VRZHESHCH, PV
    ERSHOV, DE
    ZAITSEV, SV
    VARFOLOMEEV, SD
    MYASOEDOV, NF
    BIOORGANICHESKAYA KHIMIYA, 1988, 14 (08): : 1075 - 1085
  • [36] SYNTHESIS AND EVALUATION OF BIOLOGICAL-ACTIVITY OF 2 NOVEL TRITIUM-LABELED DERIVATIVES OF RIODIPINE
    SHEVCHENKO, VP
    MYASOEDOV, NF
    SOLDATOV, NM
    DUBURS, GJ
    KASTRON, VV
    SKRASTINS, IP
    JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 1989, 27 (06): : 721 - 730
  • [37] SYNTHESIS OF TRITIUM-LABELED 14ALPHA-METHYL-5ALPHA-CHOLEST-7-EN-3BETA-OL AND ITS ENZYMATIC DEMETHYLATION
    KNIGHT, JC
    KLEIN, PD
    SZCZEPANIK, PA
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1966, 241 (07) : 1502 - +
  • [38] ENZYMATIC CONVERSION OF PROSTAGLANDIN-D2 TO PROSTAGLANDIN-F2ALPHA
    HENSBY, CN
    PROSTAGLANDINS, 1974, 8 (05): : 369 - 375
  • [39] TOTAL SYNTHESIS OF PROSTAGLANDIN D-2 METHYL-ESTER
    REYNOLDS, DP
    NEWTON, RF
    JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1979, (24) : 1150 - 1152
  • [40] PREPARATION OF TRITIUM - LABELED OLEIC ACIDS AND PROSTAGLANDINS-E2 AND PROSTAGLANDIN-F-ALPHA - INVESTIGATION OF TRITIUM INCORPORATION INTO THE MOLECULES OF UNSATURATED FATTY-ACIDS AND PROSTAGLANDINS
    SHEVCHENKO, VP
    MYASOEDOV, NF
    BEZUGLOV, VV
    BERGELSON, LD
    KHIMIYA PRIRODNYKH SOEDINENII, 1980, (02): : 148 - 157
12345