ENZYMATIC-SYNTHESIS OF TRITIUM-LABELED PROSTAGLANDIN D-2 AND ITS CONVERSION TO OTHER PROSTAGLANDINS

被引：7
|
作者：
SHRAM, SI
LAZURKINA, TY
SHEVCHENKO, VP
NAGAEV, IY
MYASOEDOV, NF
机构：
[1] Laboratory of Isotopically Labeled Physiologically Active Compounds, Institute of Molecular Genetics Kurchatov Square, Moscow
来源：
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 1994年 / 34卷 / 04期
关键词：
TRITIUM LABELED PROSTAGLANDIN D-2; ENZYMATIC SYNTHESIS; H-3] PGD(2) DERIVATIVES; L-SELECTRIDE;
D O I：
10.1002/jlcr.2580340407
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The one-stage enzymatic synthesis of tritium-labelled prostaglandin D-2 from labelled arachidonic acid was performed by using the enzyme system PGH-synthetase/PGH-PGD-isomerase. By enzymatic and chemical transformation of [H-3]PGD(2) the following compounds were obtained: 15-keto-13, 14-dihydro-[H-3]PGD(2), 9 alpha,11 beta-[H-3]PGF(2), 9-deoxy-Delta(9)-[H-3]-PGD(2) ([H-3]PGJ(2)) and Delta(12)-13, 14-dihydro-[H-3]PGJ(2). It was found that L-selectride is a more effective reducing agent than sodium borohydride in the synthesis of 9 alpha,11 beta-[H-3]PGF(2).
引用
收藏
页码：359 / 366
页数：8
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