BACULOVIRUS EXPRESSION OF THE NUCLEOCAPSID PROTEIN OF A PUUMALA SEROTYPE HANTAVIRUS

被引：5
作者：
SCHULDT, C
ZOLLER, L
BAUTZ, EKF
DARAI, G
机构：
[1] UNIV HEIDELBERG,INST MED VIROL,D-69120 HEIDELBERG,GERMANY
[2] UNIV HEIDELBERG,INST MOLEK GENET,D-69120 HEIDELBERG,GERMANY
[3] ERNST RODENWALDT AF INST MED & HYG,D-56068 KOBLENZ,GERMANY
来源：
VIRUS GENES | 1994年 / 8卷 / 02期
关键词：
BUNYAVIRIDAE; HANTAVIRUS; BACULOVIRUS; NUCLEOCAPSID PROTEIN;
D O I：
10.1007/BF01703071
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Recombinant baculoviruses were generated harboring the entire coding region of the S segment cDNA of Hantavirus strain CG 18-20 that belongs to the Puumala serotype. The recombinant nucleocapsid protein was expressed in Sf9 cells and shown to be antigenically identical with the authentic viral nucleocapsid protein by means of immunoblot analysis. Acute-phase and convalescent sera from European HERS patients recognized the recombinant nucleocapsid protein in Western blots and the recombinant Baculovirus in indirect immunofluorescence assays. Insect cells infected with the recombinant Baculoviruses proved to be a suitable noninfectious substitute for Hantavirus-infected Vero E6 cells as an antigen source for immunodiagnostic assays allowing the detection of antibodies in HFRS patients.
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页码：143 / 149
页数：7
相关论文
共 29 条
[1]   COMPARISON OF THE DEDUCED GENE-PRODUCTS OF THE L-GENOME, M-GENOME AND S-GENOME SEGMENTS OF HANTAVIRUSES [J].
ANTIC, D ;
KANG, CY ;
SPIK, K ;
SCHMALJOHN, C ;
VAPALAHTI, O ;
VAHERI, A .
VIRUS RESEARCH, 1992, 24 (01) :35-46
[2]   CODING PROPERTIES OF THE S-GENOME AND THE M-GENOME SEGMENTS OF SAPPORO RAT VIRUS - COMPARISON TO OTHER CAUSATIVE AGENTS OF HEMORRHAGIC-FEVER WITH RENAL SYNDROME [J].
ARIKAWA, J ;
LAPENOTIERE, HF ;
IACONOCONNORS, L ;
WANG, M ;
SCHMALJOHN, CS .
VIROLOGY, 1990, 176 (01) :114-125
[3]   LEAKEY VIRUS - A NEW HANTAVIRUS ISOLATED FROM MUS-MUSCULUS IN THE UNITED-STATES [J].
BAEK, LJ ;
YANAGIHARA, R ;
GIBBS, CJ ;
MIYAZAKI, M ;
GAJDUSEK, DC .
JOURNAL OF GENERAL VIROLOGY, 1988, 69 :3129-3132
[4]   EXPRESSION OF THE LASSA VIRUS NUCLEOCAPSID PROTEIN IN INSECT CELLS INFECTED WITH A RECOMBINANT BACULOVIRUS - APPLICATION TO DIAGNOSTIC ASSAYS FOR LASSA VIRUS-INFECTION [J].
BARBER, GN ;
CLEGG, JCS ;
LLOYD, G .
JOURNAL OF GENERAL VIROLOGY, 1990, 71 :19-28
[5]   AN IMMUNOFLUORESCENCE ASSAY FOR THE DETECTION OF PARVOVIRUS-B19 IGG AND IGM ANTIBODIES BASED ON RECOMBINANT VIRAL-ANTIGEN [J].
BROWN, CS ;
VANBUSSEL, MJAWM ;
WASSENAAR, ALM ;
VANELSACKERNIELE, AMW ;
WEILAND, HT ;
SALIMANS, MMM .
JOURNAL OF VIROLOGICAL METHODS, 1990, 29 (01) :53-62
[6]   SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].
CHEN, EY ;
SEEBURG, PH .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170
[7]   BELGRADE VIRUS - A NEW HANTAVIRUS CAUSING SEVERE HEMORRHAGIC-FEVER WITH RENAL SYNDROME IN YUGOSLAVIA [J].
GLIGIC, A ;
DIMKOVIC, N ;
XIAO, SY ;
BUCKLE, GJ ;
JOVANOVIC, D ;
VELIMIROVIC, D ;
STOJANOVIC, R ;
OBRADOVIC, M ;
DIGLISIC, G ;
MICIC, J ;
ASHER, DM ;
LEDUC, JW ;
YANAGIHARA, R ;
GAJDUSEK, DC .
JOURNAL OF INFECTIOUS DISEASES, 1992, 166 (01) :113-120
[8]   EPIDEMIC HEMORRHAGIC-FEVER WITH RENAL SYNDROME IN YUGOSLAVIA, 1986 [J].
GLIGIC, A ;
OBRADOVIC, M ;
STOJANOVIC, R ;
VUJOSEVIC, N ;
OVCARIC, A ;
FRUSIC, M ;
GIBBS, CJ ;
CALISHER, CH ;
GAJDUSEK, DC .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1989, 41 (01) :102-108
[9]   SERUM ANTIBODIES TO STRUCTURAL PROTEINS OF HANTAVIRUS ARISE AT DIFFERENT TIMES AFTER INFECTION [J].
GROEN, J ;
DALRYMPLE, J ;
FISHERHOCH, S ;
JORDANS, JGM ;
CLEMENT, JP ;
OSTERHAUS, ADME .
JOURNAL OF MEDICAL VIROLOGY, 1992, 37 (04) :283-287
[10]  
KYSEANDERSEN J, 1984, J BIOCHEM BIOPH METH, V10, P203
123