REACTION-MECHANISM OF PYROPHOSPHATE-DEPENDENT PHOSPHOFRUCTOKINASE FROM PINEAPPLE LEAVES

被引:0
作者
CHO, YK [1 ]
机构
[1] CHANG WON NATL UNIV,COLL NAT SCI,DEPT FOOD & NUTR,ENZYME BIOCHEM LAB,KYUNG NAM 641773,SOUTH KOREA
关键词
D O I
10.1111/j.1745-4514.1993.tb00862.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Data obtained from initial velocity, product inhibition and dead-end inhibition patterns indicate that the pyrophosphate-dependent phosphofructokinase from pineapple leaves has a rapid equilibrium random mechanism with E:F6P:MgPP(i) and E:MgPP(i):FBP dead-end complexes. The pH dependence of enzyme-reactant dissociation constants suggests that phosphates of F6P (fructose 6-phosphate), FBP (fructose 1, 6-bisphosphate), P(i) and PP(i) must be completely ionized and the lysine is present in the vicinity of the 1- and 6-phosphates of the sugar phosphate and bisphosphate. The pH dependence of kinetic parameters suggests that the enzyme catalyzes the reaction via general acid-base catalysis with the use of a proton shuttle. The base is required to be unprotonated in both reaction directions.
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页码:69 / 83
页数:15
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