BOTH ANKYRIN AND BAND 4.1 ARE REQUIRED TO RESTRICT THE ROTATIONAL MOBILITY OF BAND-3 IN THE HUMAN ERYTHROCYTE-MEMBRANE

被引：15

作者：

WYATT, K ^{[1
]}

CHERRY, RJ ^{[1
]}

机构：

[1] UNIV ESSEX,DEPT CHEM & BIOL CHEM,WIVENHOE PK,COLCHESTER CO4 3SQ,ESSEX,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1103卷 / 02期

关键词：

CYTOSKELETON; TRANSIENT DICHROISM; TRIPLET PROBE;

D O I：

10.1016/0005-2736(92)90104-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A population of band 3 proteins in the human erythrocyte membrane is known to have restricted rotational mobility due to interaction with cytoskeletal proteins. We have further investigated the cause of this restriction by measuring the effects on band 3 rotational mobility of rebinding ankyrin and band 4.1 to ghosts stripped of these proteins as well as spectrin and actin. Rebinding either ankyrin or 4.1 alone has no detectable effect on band 3 mobility. Rebinding both these proteins together does, however, reimpose a restriction on band 3 rotation. The effect on band 3 rotational mobility of rebinding ankyrin and 4.1 are similar irrespective of whether or not band 4.2 is removed from the membrane. We suggest that ankyrin and 4.1 together promote the formation of slowly rotating clusters of band 3.

引用

页码：327 / 330

页数：4

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