ISOLATION AND MONOLAYER-CULTURE OF HUMAN FALLOPIAN-TUBE EPITHELIAL-CELLS

被引：0

作者：

TAKEUCHI, K ^{[1
]}

MARUYAMA, I ^{[1
]}

YAMAMOTO, S ^{[1
]}

OKI, T ^{[1
]}

NAGATA, Y ^{[1
]}

机构：

[1] KAGOSHIMA UNIV,DEPT INTERNAL MED,KAGOSHIMA 890,JAPAN

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY | 1991年 / 27卷 / 09期

关键词：

HUMAN TUBAL EPITHELIUM; MONOLAYER CELL CULTURE; EPIDERMAL GROWTH FACTOR;

D O I：

暂无

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In the present study we have established a pure monolayer culture system of human fallopian tube epithelial cells. The cells were isolated using collagenase digestion, and were cultured in Medium 199 supplemented with 15% fetal bovine serum. The epithelial cells derived from primary and secondary culture were characterized using immunocytochemical staining and electron microscopy. The cells continued to grow for 2 to 3 wk once the monolayer culture of the cells was established. It is currently possible to maintain the cultures until the third generation. Proliferation of these cells was enhanced by epidermal growth factor but not by basic-fibroblast growth factor, insulin, transferrin, estradiol-17-beta, or progesterone. This culture system offers a good model for determining characteristics of the tubal epithelium and would permit effective study of co-culture with embryos.

引用

页码：720 / 724

页数：5

共 18 条

[1] EPIDERMAL GROWTH-FACTOR RECEPTOR EXPRESSION IN NORMAL AND MALIGNANT ENDOMETRIUM [J].