CHIRAL SEPARATIONS BY CAPILLARY ELECTROPHORESIS USING CYCLODEXTRIN-CONTAINING GELS

Allyl carbamoylated beta-cyclodextrin derivatives were synthesized to be used as chiral resolving agents when copolymerized with acrylamide to form gels suitable for enantiomer separations in capillary electrophoresis. Both solid and liquid gels have been produced by adjusting the concentrations of acrylamide, bis-acrylamide and the allyl cyclodextrin derivative. The liquid gels were free of the problems associated with solid gels: bubble formation, short lifetime and poor reproducibility. The chiral selectivity of the liquid cyclodextrin gels in the separation of dansylated amino acid enantiomers depended on the cyclodextrin concentration of the gel. The liquid gels were successfully used to separate the enantiomers of several chiral molecules.