EFFECT OF HYLAN ON CARTILAGE AND CHONDROCYTE CULTURES

被引:105
作者
LARSEN, NE [1 ]
LOMBARD, KM [1 ]
PARENT, EG [1 ]
BALAZS, EA [1 ]
机构
[1] UNIV CONNECTICUT,DEPT TOXICOL,STORRS,CT 06268
关键词
HYALIN; HYALURONAN; ARTICULAR CARTILAGE; CHONDROCYTES; PROTEOGLYCAN; POLYMORPHONUCLEAR LEUKOCYTES; OXYGEN-DERIVED FREE RADICALS;
D O I
10.1002/jor.1100100104
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
The protective role of hylan, a hyaluronan [hyaluronic acid (HA)] derivative, was studied in explanted bovine cartilage and isolated chondrocytes. Cartilage and chondrocytes were exposed to degradative enzymes (lysate from activated polymorphonuclear leukocytes), oxygen-derived free radicals (ODFR), conditioned media from mononuclear cells (MCCM), and interleukin-1 (IL-1), in the presence and absence of hylan. The effect of HA was also studied. In cartilage explants susceptibility to pertubation was evaluated in terms of S-35 release and proteoglycan depletion and was compared to control cultures; high viscosity hylan was found to reduce S-35 release in cartilage explants caused by degradative enzymes, ODFR, MCCM, and IL-1. The hylan effect was reversible and viscosity-dependent. In chondrocyte cultures, high viscosity hylan was effective in reducing cell injury caused by degradative enzymes and ODFR. The data suggest that the glycosaminoglycan hylan, as well as native HA, may mediate exposure to and/or response to stimuli associated with initiation of degenerative processes in cartilage tissues.
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页码:23 / 32
页数:10
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