NUCLEOTIDE-SEQUENCE OF REGION PRECEDING TRP MESSENGER-RNA INITIATION SITE AND ITS ROLE IN PROMOTER AND OPERATOR FUNCTION

被引：88

作者：

BENNETT, GN ^{[1
]}

SCHWEINGRUBER, ME ^{[1
]}

BROWN, KD ^{[1
]}

SQUIRES, C ^{[1
]}

YANOFSKY, C ^{[1
]}

机构：

[1] STANFORD UNIV, DEPT BIOL SCI, STANFORD, CA 94305 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1976年 / 73卷 / 07期

关键词：

D O I：

10.1073/pnas.73.7.2351

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The nucleotide sequence of the region preceding the transcription initiation site of the tryptophan operon of Escherichia coli was determined. Essentially all of the trp operator precedes the transcribed portion of the operon. The deduced sequence contains the recognition site of [restriction] endonuclease Hpa I. This site is protected from Hpa I cleavage by RNA polymerase and by trp repressor. Regions of 2-fold symmetry are present in the DNA sequence.

引用

页码：2351 / 2355

页数：5

共 27 条

[1]

BARRELL BG, 1971, PROCEDURES NUCLEIC A, V2, P751

[2]

BERTRAND K, IN PRESS

[3] NUCLEOTIDE SEQUENCES FROM TRYPTOPHAN MESSENGER-RNA OF ESCHERICHIA-COLI - SEQUENCE CORRESPONDING TO AMINO-TERMINAL REGION OF FIRST POLYPEPTIDE SPECIFIED BY OPERON [J].