THE PARAMYXOVIRUS SV5 V-PROTEIN BINDS 2 ATOMS OF ZINC AND IS A STRUCTURAL COMPONENT OF VIRIONS

被引：125

作者：

PATERSON, RG ^{[1
]}

LESER, GP ^{[1
]}

SHAUGHNESSY, MA ^{[1
]}

LAMB, RA ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,HOWARD HUGHES MED INST,EVANSTON,IL 60208

来源：

VIROLOGY | 1995年 / 208卷 / 01期

关键词：

D O I：

10.1006/viro.1995.1135

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The paramyxovirus simian virus 5 (SV5) cysteine-rich V protein has been shown to be a virus structural protein by analysis of the polypeptides of purified SV5 virions. In addition, the V protein has been identified as a component of the virus nucleocapsid core both by the analysis of the polypeptides present in radioactively labeled preparations of purified nucleocapsids and by immunoelectron microscopy. Quantitative autoradiography was used to determine that there are similar to 350 molecules of the V protein in virions. The V protein has been purified from V recombinant baculovirus-infected insect cells and by using inductively coupled argon plasma atomic emission spectroscopy it was found that each molecule of V binds two zinc atoms. (C) 1995 Academic Press, Inc.

引用

页码：121 / 131

页数：11

共 34 条

[1]

BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5

[2] THE RULE OF 6, A BASIC FEATURE FOR EFFICIENT REPLICATION OF SENDAI VIRUS DEFECTIVE INTERFERING RNA [J].

CALAIN, P ;

ROUX, L .

JOURNAL OF VIROLOGY, 1993, 67 (08) :4822-4830

[3] MULTIPLICATION OF MYXOVIRUS ( SV5 ) WITH MINIMAL CYTOPATHIC EFFECTS + WITHOUT INTERFERENCE [J].

CHOPPIN, PW .

VIROLOGY, 1964, 23 (02) :224-&

[4] CHARACTERIZATION OF THE SENDAI VIRUS V-PROTEIN WITH AN ANTIPEPTIDE ANTISERUM [J].

CURRAN, J ;

DEMELO, M ;

MOYER, S ;

KOLAKOFSKY, D .

VIROLOGY, 1991, 184 (01) :108-116

[5] THE SENDAI VIRUS P-GENE EXPRESSES BOTH AN ESSENTIAL PROTEIN AND AN INHIBITOR OF RNA-SYNTHESIS BY SHUFFLING MODULES VIA MESSENGER-RNA EDITING [J].

CURRAN, J ;

BOECK, R ;

KOLAKOFSKY, D .

EMBO JOURNAL, 1991, 10 (10) :3079-3085

[6] AN ACIDIC ACTIVATION-LIKE DOMAIN OF THE SENDAI VIRUS-P PROTEIN IS REQUIRED FOR RNA-SYNTHESIS AND ENCAPSIDATION [J].

CURRAN, J ;

PELET, T ;

KOLAKOFSKY, D .

VIROLOGY, 1994, 202 (02) :875-884

[7] THE SENDAI VIRUS NUCLEOCAPSID EXISTS IN AT LEAST 4 DIFFERENT HELICAL STATES [J].

EGELMAN, EH ;

WU, SS ;

AMREIN, M ;

PORTNER, A ;

MURTI, G .

JOURNAL OF VIROLOGY, 1989, 63 (05) :2233-2243

[8]

HARLOW E, 1988, ANTIBODIES LABORATOR

[9] IDENTIFICATION AND PREDICTED SEQUENCE OF A PREVIOUSLY UNRECOGNIZED SMALL HYDROPHOBIC PROTEIN, SH, OF THE PARAMYXOVIRUS SIMIAN VIRUS-5 [J].

HIEBERT, SW ;

PATERSON, RG ;

LAMB, RA .

JOURNAL OF VIROLOGY, 1985, 55 (03) :744-751

[10] PSEUDO-TEMPLATED TRANSCRIPTION IN PROKARYOTIC AND EUKARYOTIC ORGANISMS [J].

JACQUES, JP ;

KOLAKOFSKY, D .

GENES & DEVELOPMENT, 1991, 5 (05) :707-713

← 1 2 3 4 →