FERTILIZATION RATE IN SHEEP UNILATERALLY INSEMINATED WITH FROZEN-SEMEN

被引:7
作者
CORREA, JE
BERGMANN, B
GATICA, R
机构
[1] Animal Reproduction Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia
关键词
SHEEP; ARTIFICIAL INSEMINATION; FROZEN SEMEN; FERTILIZATION RATE; SURGICAL TECHNIQUE;
D O I
10.1016/0921-4488(94)90037-X
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
In order to evaluate a simple surgical technique for artificial insemination (AI) with frozen semen, 40 adult ewes were synchronised with vaginal sponges impregnated with 60 mg of medroxyprogesterone acetate for 12 d and a single injection of 400 IU PMSG at sponge withdrawal. Semen from fertile rams was pooled and frozen in straws, each containing 3 x 10(8) spermatozoa. All ewes were inseminated 60 h after PMSG injection. Twenty ewes (group A) were unilaterally inseminated by a small midventral laparotomy and 20 (group B) were cervically inseminated. Fertilization rate was measured by flushing of uteri and oviducts throughout a laparotomy carried out 3-4 d after insemination. The 83.7% fertilization rate in group A was significantly different (P < 0.0001) than the 19.1% fertilization rate of group B. This surgical technique seems to be useful when no laparoscopic equipment is available.
引用
收藏
页码:99 / 101
页数:3
相关论文
共 16 条
  • [1] Correa, Embryo mortality in Corriedale ewes in Tierra del Fuego (Chile), Proc. VIII International Congress on Animal Reproduction and Artificial Insemination. Munich, Germany, 2, pp. 1163-1168, (1972)
  • [2] Correa, Use of mass laparotomy for an egg transfer technique to the uterus of the ewe, Vet. Rec., 99, (1976)
  • [3] Eppleston, Roberts, The effect of progestagen, PMSG and time of insemination on fertility in ewes following intra-uterine insemination with frozen semen, Aust. Vet. J., 63, pp. 124-125, (1986)
  • [4] First, Sevinge, Henneman, Fertility of frozen and unfrozen ram semen, J. Anim. Sci., 20, pp. 79-84, (1961)
  • [5] Halbert, Dobson, Walton, Sharpe, Buckrell, Field evaluation of a technique for transcervical intrauterine insemination of ewes, Theriogenology, 33, pp. 1231-1243, (1990)
  • [6] Hellemann, Gonzalez, Guzman, Efecto de yema de huevo en polvo, un surfactante y centrifugacíon en la sobrevivencia de espermatozoídes caprinos congelados, Arch. Med. Vet., 24, pp. 141-148, (1992)
  • [7] Iritani, Problems of freezing spermatozoa of different species, Proc. IX International Congress on Animal Reproduction and Artificial Insemination. Madrid, Spain, 1, pp. 115-132, (1980)
  • [8] Killen, Caffery, Uterine insemination of ewes with the aid of a laparoscope, Aust. Vet. J., 59, (1982)
  • [9] Lightfoot, Salamon, Fertility of ram spermatozoa frozen by the pellet method II The effects of method of insemination on fertilization and embryonic mortality, Reproduction, 22, pp. 339-408, (1970)
  • [10] Maxwell, Wilson, Butler, Fertility of ewes after intrauterine insemination with frozen semen, Anim. Prod. Aust., 15, pp. 148-158, (1984)