CHARACTERIZATION OF A HIGH MOLECULAR MASS CYTOKININ-LIKE COMPOUND EXTRACTED FROM WHEAT-GERM (TRITICUM-VULGARE L)

被引：3
|
作者：
PAPET, MP
FOURNET, B
MONSIGNY, M
DELMOTTE, F
机构：
[1] CNRS,CTR BIOPHYS MOLEC,DEPT BIOCHIM GLYCOCONJUGUES & LECTINES ENDOGENES,1 RUE HAUTE,F-45045 ORLEANS,FRANCE
[2] UNIV ORLEANS,F-45071 ORLEANS 2,FRANCE
来源：
PLANT SCIENCE | 1990年 / 68卷 / 02期
关键词：
cytokinin; glycoside; growth regulator; oligosaccharide; wheat germ;
D O I：
10.1016/0168-9452(90)90222-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe the presence of a glycosyl derivative from wheat germ with a cytokinin activity. This compound was isolated after extraction by four chromatography steps: sulfopropyl ion exchange, Sephadex LH-20, Trisacryl GF 05 gel filtration and cellulose thin-layer chromatography. Glucose and xylose were identified by gas chromatography in the ratio 2:1. This glycosylated derivative has a maximal absorption at 277 nm due to an expected aglycone. After peracetylation, the molecular mass obtained in FAB mass spectrometry was 1333 in positive mode (M + H)+ and 1331, in negative mode (M - H)-. This compound showed an optimal activity on the cucumber cotyledon assay at 4 × 10-5 M equivalent concentration of 6-benzylaminopurine. © 1990.
引用
收藏
页码：175 / 182
页数：8
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