MANIPULATION OF INTRACELLULAR MAGNESIUM CONTENT IN POLYMYXIN-B NONAPEPTIDE-SENSITIZED ESCHERICHIA-COLI BY IONOPHORE A23187

被引：163

作者：

ALATOSSAVA, T ^{[1
]}

JUTTE, H ^{[1
]}

KUHN, A ^{[1
]}

KELLENBERGER, E ^{[1
]}

机构：

[1] UNIV BASEL, DEPT MICROBIOL, BIOZENTRUM, CH-4056 BASEL, SWITZERLAND

来源：

JOURNAL OF BACTERIOLOGY | 1985年 / 162卷 / 01期

关键词：

D O I：

10.1128/JB.162.1.413-419.1985

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

E. coli B cells were sensitized to ionophore A23187 by polymyxin B nonapeptide, and the induced Mg and K ion fluxes were studied. Combined ionophore treatment permeabilized the cytoplasmic membrane of E. coli in an ion-specific manner and allowed the manipulation of intracellular Mg2+ content from the outside. A23187-induced Mg2+ efflux or influx was dependent on the free Mg2+ concentration gradient between the outside and inside of the cytoplasmic membrane and on the pH gradient. Most of the intracellular Mg2+ was bound, whereas only 1-2 mM was free in solution in the cellular sap.

引用

页码：413 / 419

页数：7

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