BIOCHEMICAL-ALTERATIONS IN MULTIPLE-SCLEROSIS LESIONS AND NORMAL-APPEARING WHITE-MATTER DETECTED BY IN-VIVO P-31 AND H-1 SPECTROSCOPIC IMAGING

The goals of the current study were threefold: first, to confirm previous single volume proton (H-1) magnetic resonance spectroscopy results of reduced N-acetyl aspartate (NAA, a putative marker of neurons) in multiple sclerosis (MS) white matter lesions using multiple volume H-1 magnetic resonance spectroscopic imaging (MRSI); second, to measure the phospholipid metabolites phosphomonoesters and phosphodiesters in such lesions using phosphorus (P-31) MRSI; and third, to test the hypothesis that biochemical changes occur in the normal-appearing (on spin echo T2-weighted magnetic resonance images) white matter in patients with MS. Thirteen subjects with clinically definite MS were studied with both H-1 and P-31 MRSI, and 19 controls were studied with either H-1 MRSI, P-31 MRSI, or both. MS lesion, MS normal-appearing white matter, and region-matched control spectra from the centrum semiovale were analyzed. The major findings of this study were that in both white matter lesions and normal-appearing white matter in patients with MS, the metabolite ratio NAA/creatine and the total P-31 peak integrals were significantly reduced compared with controls. In addition, in MS lesions NAA/choline and phosphodiesters/total P-31 were significantly reduced compared with controls, and in MS normal-appearing white matter there was a trend for NAA/choline to be reduced compared with controls. In normal-appearing white matter in patients with MS, total creatine and phosphocreatine were significantly increased compared to controls, as detected with both H-1 (total creatine peak integrals) and P-31 (phosphocreatine/total P-31) MRSI techniques. These results suggest reduced neuronal density and altered phospholipid metabolites in white matter lesions in patients with MS. Furthermore, the results suggest the presence of biochemical abnormalities not detected by standard spin echo magnetic resonance imaging in normal-appearing white matter in MS.