SYNTHESIS OF A FRAGMENT OF THYMOSIN-BETA-10 FOR THE DEVELOPMENT OF SPECIFIC ANTIBODIES

被引：0
作者：
HORGER, S
GALLERT, B
ECHNER, H
VOELTER, W
机构：
[1] UNIV TUBINGEN,INST PHYSIOL,PHYS BIOCHEM ABT,HOPPE SEYLER STR 4,W-7400 TUBINGEN 1,GERMANY
[2] UNIV TUBINGEN,INST CHEM,PHYS BIOCHEM ABT,W-7400 TUBINGEN 1,GERMANY
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES | 1992年 / 47卷 / 08期
关键词：
THYMOSIN-BETA-10; ANTIBODY DEVELOPMENT; SOLID PHASE PEPTIDE SYNTHESIS;
D O I：
暂无
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
The N-terminal fragment 1 - 12 of thymosin-beta-10 was synthesized by the solid phase method using p-benzyloxybenzyl alcohol/polystyrene/divinylbenzeneresin and N-alpha-Fmoc amino acids with tert-butyl or Boc side chain protecting groups. Coupling was performed with BOP. The peptide was purified by preparative HPLC.
引用
收藏
页码：1170 / 1174
页数：5
相关论文
共 14 条
[1]  
Chou P Y, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P45
[2]   THYMOSIN-BETA-10, A NEW ANALOG OF THYMOSIN-BETA-4 IN MAMMALIAN-TISSUES [J].
ERICKSONVIITANEN, S ;
RUGGIERI, S ;
NATALINI, P ;
HORECKER, BL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 225 (02) :407-413
[3]   MOLECULAR-CLONING OF THE CDNA FOR RAT SPLEEN THYMOSIN BETA-10 AND THE DEDUCED AMINO-ACID-SEQUENCE [J].
GOODALL, GJ ;
HORECKER, BL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 256 (01) :402-405
[4]   DEVELOPMENTAL REGULATION OF THYMOSIN-BETA-10 MESSENGER-RNA IN THE HUMAN BRAIN [J].
HALL, AK .
MOLECULAR BRAIN RESEARCH, 1991, 9 (1-2) :175-177
[5]  
JAMESON BA, 1988, COMPUT APPL BIOSCI, V4, P181
[6]   COLOR TEST FOR DETECTION OF FREE TERMINAL AMINO GROUPS IN SOLID-PHASE SYNTHESIS OF PEPTIDES [J].
KAISER, E ;
COLESCOT.RL ;
BOSSINGE.CD ;
COOK, PI .
ANALYTICAL BIOCHEMISTRY, 1970, 34 (02) :595-&
[7]   DEVELOPMENTAL EXPRESSION OF MESSENGER-RNAS ENCODING THYMOSIN-BETA-4 AND THYMOSIN-BETA-10 IN RAT-BRAIN AND OTHER TISSUES [J].
LIN, SC ;
MORRISONBOGORAD, M .
JOURNAL OF MOLECULAR NEUROSCIENCE, 1990, 2 (01) :35-44
[8]   A THYMOSIN-BETA-4 ELISA USING AN ANTIBODY AGAINST THE N-TERMINAL FRAGMENT THYMOSIN-BETA-4 [1-14] [J].
LIVANIOU, E ;
MIHELIC, M ;
EVANGELATOS, GP ;
HARITOS, A ;
VOELTER, W .
JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 148 (1-2) :9-14
[9]   IMPROVED SYNTHESIS OF 4-ALKOXYBENZYL ALCOHOL RESIN [J].
LU, G ;
MOJSOV, S ;
TAM, JP ;
MERRIFIELD, RB .
JOURNAL OF ORGANIC CHEMISTRY, 1981, 46 (17) :3433-3436
[10]   MICROELISA METHOD FOR THE DETERMINATION OF THYMOSIN-BETA-9 DISCRIMINATING BETWEEN THYMOSIN-BETA-9 AND THE STRUCTURALLY CLOSELY RELATED THYMOSIN-BETA-4 [J].
MIHELIC, M ;
KALBACHER, H ;
HANNAPPEL, E ;
VOELTER, W .
JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 122 (01) :7-13
12