PRODUCTS OF OXIDATIVE COUPLING OF PHENOL BY HORSERADISH-PEROXIDASE

The oxidation and coupling of phenol by horseradish peroxidase in the presence of hydrogen peroxide yielded dimers trimers and higher molecular weight polymers which are mostly insoluble in water, so the products may be removed by centrifugation. However we discovered some products, such as dimers o,o'-biphenol, p,p'-biphenol,o,p'-biphenol,o-phenoxyphenol and p-phenoxyphenol, even though trimer 4-(4-phenoxyphenoxy)phenol were present in the aqueous phase at a very low concentration.